amphotericin-b and Poisoning

To find novel amphotericin B (AmB) derivatives with high therapeutic potential, low toxicity, and water solubility, a series of nine N-substituted AmB derivatives were evaluated for their antifungal activity using the broth dilution method and for their hemolytic toxicity with sterile defibrinated sheep blood. Qualitative screening of the effect of the derivatives on two reference Candida albicans strains and of their solubility was performed based on the value of n (n is a positive integer), resulting in the identification of an optimal compound, NH

Topics: Amphotericin B; Animals; Antifungal Agents; Candida albicans; Cell Survival; Cytological Techniques; Disease Models, Animal; Erythrocytes; HEK293 Cells; Hemolysis; Humans; Microbial Sensitivity Tests; Poisoning; Sheep; Solubility

Amphotericin B (1mg/kg/day) was administered intravenously to rats for 30 days, with simultaneous sham (5% dextrose in saline) injections to control animals. After four treatments, serum cholesterol concentration among treated animals demonstrated a maximum decrease of 25% (p less than 0.005) from the control level. Serum cholesterol returned to control level after three more treatments, and no significant difference (p greater than 0.05) was observed in experimental animals, despite continued treatment thereafter. Various hematological and biochemical parameters were monitored at intervals during this period. Except for significantly elevated (p less than 0.001) plasma urea nitrogen in treated animals, no significant difference was observed between the control and treated animals with regard to other parameters. Gross examination at autopsy, followed by microscopic study, showed absence of organ pathology.

Topics: Amphotericin B; Animals; Blood Cell Count; Cholesterol; Hemoglobinometry; Male; Poisoning; Rats; Time Factors

Topics: Amphotericin B; Anti-Bacterial Agents; Aspergillosis; Humans; Lung Diseases; Lung Diseases, Fungal; Nervous System Diseases; Neurologic Manifestations; Peripheral Nervous System Diseases; Poisoning; Toxicology