amphotericin-b and Eye-Infections

The aim of this study is to identify the pathogen causing ocular infection in a Chinese patient and to describe its morphological characteristics.. Samples from the patient's intraoperative pus were collected for microscopic examination and culture. Morphology and drug sensitivities of the isolated fungus were analyzed. Ribosomal DNA (rDNA) sequencing was performed and blasted in GenBank.. The case was infected with

Topics: Amphotericin B; Ascomycota; Eye Infections; Humans; Phylogeny

Members of genus Rhodotorula are widely distributed in nature and have been traditionally considered non-pathogenic. Last few decades have seen the yeast as an emerging pathogen. We observed increase in numbers of Rhodotorula isolates from ocular infections in last few years, thus this prospective study was planned.. To identify the species of Rhodotorula isolates from ocular infections. To know the antifungal susceptibilities and study the biofilm formation attributes of the isolates.. Rhodotorula isolates were speciated using conventional methods, Matrix Assisted Laser Desorption and Ionisation - Time of Flight (MALDI- TOF) and sequencing of ITS region of ribosomal DNA. Antifungal susceptibility testing (AFST) was done using disc diffusion and E-test. Biofilm formation was studied using XTT [2,3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetra-zolium-5-carboxanilide] assay.. Twenty four isolates (92.3%) were identified as R. mucilaginosa and two as R. Minuta. AFST showed high MICs against Fluconazole, Amphotericin-B, Caspofungin, Micafungin and Flucytosine; MIC distribution from low to very high against Voriconazole, Itraconazole and Natamycin; and very low MICs against Posaconazole 57.7% of isolates were strong biofilm producers, 23.1% were moderate, and 19.2% were non producers.. This is the first prospective study on species distribution, antifungal susceptibility and biofilm production attributes of Rhodotorula isolates from ocular infections; also first time demonstrating the utility of proteomics based MALDI-TOF in diagnosing Rhodotorula up to species level. The study has shown high MICs against the conventional azoles, Amphotericin-B and Flucytosine. However, low MICs against Posaconazole and Natamycin give a hope for their possible therapeutic use.

Topics: Amphotericin B; Antifungal Agents; Biofilms; Eye Infections; Flucytosine; Humans; Microbial Sensitivity Tests; Natamycin; Prospective Studies; Rhodotorula

Topics: Amphotericin B; Blood; Choroiditis; Eye Infections; Fatal Outcome; Functional Laterality; Humans; Male; Middle Aged; Prototheca

Topics: Acremonium; Aged; Amphotericin B; Antifungal Agents; Cornea; Delftia acidovorans; Eye Infections; Eye Infections, Bacterial; Eye Infections, Fungal; Female; Gram-Negative Bacterial Infections; Humans; Keratitis; Male; Microsporum; Middle Aged; Mycoses; Natamycin; Ophthalmic Solutions