amphotericin-b and Cell-Transformation--Neoplastic

Topics: Aged; Amphotericin B; Antifungal Agents; Bone Marrow; Cell Transformation, Neoplastic; Histoplasmosis; Humans; Itraconazole; Leukemia, Lymphocytic, Chronic, B-Cell; Liver; Lymph Nodes; Lymphoma, Large B-Cell, Diffuse; Male; Radionuclide Imaging; Spleen; Treatment Outcome

Topics: Amphotericin B; Animals; Cell Division; Cell Transformation, Neoplastic; Deoxycholic Acid; Dose-Response Relationship, Drug; Melanoma, Experimental; Mice; Monophenol Monooxygenase; Tumor Cells, Cultured

Study of an experimental neoplastic process induced by virus type strains of Rous's sarcoma has shown that amphotericin B potentiates antineoplastic action of cyclophosphamide. Administration of cytostatin does not affect the fractional composition of chromatin proteins. Administration of polyene changes this indicator, the changes being greater after combined use of both drugs. The character of a considerable part of the abnormalities in the fractional composition of chromatin proteins indicates that chromatin had been exposed to proteolytic enzymes.

Topics: Amphotericin B; Animals; Avian Sarcoma Viruses; Cell Transformation, Neoplastic; Chick Embryo; Chickens; Cyclophosphamide; Drug Interactions; Electrophoresis, Polyacrylamide Gel; Nucleoproteins; Sarcoma, Avian

The imidazole antimycotics clotrimazole and miconazole were tested on untransformed rat cell line 3Y1-B clone 1-6 (3Y1) and six transformed cell lines, which were independently isolated from 3Y1 or 3Y1-B clone 1 after infection with adenovirus 12 (AD-12) or with SV40, to determine their sensitivities to these drugs. The relative plating efficiency of three cell lines (T3, W4, and W5) transformed with AD-12 was reduced to 10(-1( of the initial value by clotrimazole (2 to 4 microgram/ml), whereas that of the parental cell line 3Y1 was reduced to 10(-1) of the initial value by clotrimazole (20 to 25 microgram/ml). By contrast, the differential effect of miconazole on 3Y1 and AD-12-transformed cell lines was found to be a factor of 2. The sensitivity of the SV40-transformed 3Y1 and the AD-12-transformed cell lines. The cellular sensitivity of untransformed 3Y1 cells to clotrimazole was significantly enhanced when exposed to various doses of the unsaturated fatty acid, linoleic acid. The untransformed and transformed cell lines showed sensitivities similar to the cytocidal activity of sterol-binding antimycotics, amphotericin B and filipin.

Topics: Amphotericin B; Animals; Cell Line; Cell Membrane; Cell Transformation, Neoplastic; Clotrimazole; Filipin; Imidazoles; Membrane Lipids; Miconazole; Rats

Topics: Amphotericin B; Biological Transport, Active; Blood; Blood Platelets; Cell Transformation, Neoplastic; Cells, Cultured; DNA; Fibroblasts; Growth Substances; Melitten; Monensin; Peptides; Platelet-Derived Growth Factor; Potassium; Rubidium; Sodium; Vasopressins

Several responses of 10T 1/2 cells and of cells from strains transformed by X-irradiation or by methylcholanthrene exposure were examined. Malignant potential, as determined by the ability of the cells to initiate tumors in syngeneic hosts, either normal or rendered immunodeficient, varied by a factor of 10(6). The growth rate of tumors both in normal and in irradiated animals was closely correlated to malignant potential. The life spans of the animals were inversely correlated with malignant potential. Serum requirements were high for 10T 1/2 and for one of the X-ray transformants; neither of these cell strains gave rise to tumors when cells were injected into hosts. All malignant strains had uniformly lower serum requirements. While 10T 1/2 cells tended to be somewhat more heat resistant than were any of their transformed counterparts, this depended upon cell density (or state of growth) during heating. There was no correlation between malignant potential and heat sensitivity among the transformed strains. In fact, the most resistant transformant was also the most malignant; furthermore, its heat sensitivity was not modified by growth in vivo. No relationship between malignant potential and response to the polyene antibiotic amphotericin B could be observed.

Topics: Amphotericin B; Animals; Cell Line; Cell Survival; Cell Transformation, Neoplastic; Dose-Response Relationship, Drug; Hot Temperature; Methylcholanthrene; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Neoplasms, Experimental; Transplantation, Isogeneic; X-Rays

Transformation of mouse 3T3 cells by SV40 results in a specific membrane modification which renders cells resistant to the killing action of the lipophilic antibiotic Amphotericin B. This alteration is under genetic cellular control and is specific for SV40 transformation since transformation with polyoma or mouse sarcoma viruses does not confer resistance to the antibiotic. Analogous resistance is induced by SV40 transformation of primary human fibroblast cells. The acquired resistance is not due to decreased binding of Amphotericin B and is partially reversed if cells are grown in the presence of cholesterol. The results are interpreted as a specific change of sterol structure of the membrane or the loss of a minor cholesterol fraction responsible for the killing action of the antibiotic.

Topics: Amphotericin B; Cell Line; Cell Membrane; Cell Transformation, Neoplastic; Cholesterol; Drug Resistance; Polyomavirus; Sarcoma Viruses, Murine; Simian virus 40

Reproducible X-ray-induced oncogenic transformation has been demonstrated in an established cell line of mouse embryo fibroblasts. Cells derived from transformed foci formed malignant tumors when injected into syngeneic hosts. An exponential increase in the number of transformants per viable cell occurred with doses of up to 400 rads of X-radiation. The transformation frequency in exponentially growing cultures remained constant at 2.3 x 10(-3) following doses of 400 to 1500 rads. There was little change in survival following X-ray doses up to 300 rads. Doses greater than 300 rads were associated with an exponential decline in survival; the Do for the survival curve was 175 rads. Transformation frequency varied with changes in the number of viable cells seeded per dish. There was about a 10-fold decline in the transformation frequency when the number of cells was increased from 400 to 1000 viable cells/100-mm Petri dish. Below this density range there was little change in transformation frequency. The presence of lethally preir-radiated cells was not associated with an enhancement of transformation in irradiated cells or with the induction of transformation in unirradiated cell cultures. Amphotericin B (Fungizone) inhibited the appearance of transformants when added to the culture medium within 2 to 3 weeks after initiation of the experiment.

Topics: Amphotericin B; Animals; Cell Line; Cell Survival; Cell Transformation, Neoplastic; Cells, Cultured; Dose-Response Relationship, Radiation; Fibroblasts; Mice; Mice, Inbred C3H; Neoplasms, Experimental; Radiation Effects; X-Rays

Topics: Amphotericin B; Binding Sites; Cell Division; Cell Line; Cell Membrane; Cell Survival; Cell Transformation, Neoplastic; Depression, Chemical; Dose-Response Relationship, Drug; Polyomavirus; Simian virus 40; Transcription, Genetic

Topics: Amphotericin B; Animals; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Dimethylformamide; Drug Synergism; Ethanol; Gammaretrovirus; Mice; Mice, Inbred BALB C; Rifamycins; Sarcoma, Experimental

Topics: Amphotericin B; Animals; Cell Line; Cell Transformation, Neoplastic; Cells, Cultured; Clone Cells; Contact Inhibition; Cricetinae; Drug Resistance; Fibroblasts; Humans; Hybrid Cells; Kidney; Mice; Mice, Inbred BALB C; Simian virus 40; Tritium; Uridine

By using cultured, transformed fibroblastic cells, antitumor agents including cytosine arabinoside, thio-tepa, nitrogen mustard N-oxide, mitomycin C, chromomycin A3, 5-fluorouracil, daunomycin, vinblastine, vincristine, and bleomycin A2 were screened for the potentiation of their activity by amphotericin B or polymyxin B. Among them, the action of 5-fluorouracil and chromomycin A3 on ribonucleic acid synthesis was potentiated in their action by amphotericin B, not by polymyxin B. Bleomycin A2 was potentiated in its action on deoxyribonucleic acid and ribonucleic acid synthesis only by polymyxin B.

Topics: Amphotericin B; Animals; Bleomycin; Cell Transformation, Neoplastic; Chromomycins; Drug Synergism; Fibroblasts; Fluorouracil; Polymyxins; Rats

Topics: Amphotericin B; Animals; Carbon Radioisotopes; Cell Line; Cell Survival; Cell Transformation, Neoplastic; Dose-Response Relationship, Drug; Drug Synergism; Fibroblasts; Fusidic Acid; Leucine; Neoplasm Proteins; Polymyxins; Protein Biosynthesis; Rats; RNA; RNA, Neoplasm; Tritium; Uracil

Topics: Amphotericin B; Animals; Benz(a)Anthracenes; Cell Division; Cell Line; Cell Transformation, Neoplastic; Clone Cells; Dose-Response Relationship, Drug; Embryo, Mammalian; Fibrosarcoma; Karyotyping; Methylcholanthrene; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Nitrosoguanidines; Sarcoma, Experimental; Time Factors; Transplantation, Homologous

Topics: Amphotericin B; Animals; Basidiomycota; Carbon Radioisotopes; Cell Transformation, Neoplastic; Centrifugation, Density Gradient; Dactinomycin; Depression, Chemical; Drug Synergism; Fibroblasts; Leucine; Mycotoxins; Oligopeptides; Peptides, Cyclic; Polyribosomes; Protein Biosynthesis; Rats; RNA, Messenger; RNA, Ribosomal; Time Factors; Tritium; Uridine

One of the most potent inhibitors of RNA-dependent DNA polymerase activity so far described (rifazacyclo-16) was not correspondingly as active in focus inhibition. This discrepancy was thought to be due to the inability of the drug to penetrate the cell membrane. It has been found that a very low level of amphotericin B allows this drug, as well as the previously described 2',6'-dimethyl-N(4')benzyl-N(4')-[desmethyl] rifampicin, to exhibit a very high capability to inhibit focus formation. Since these two drugs are highly lipophilie, their activity may be expected to be dependent upon any lipophilic components in the medium, such as serum or detergents. The use of amphotericin B as well as serum in tissue cultures is common, and could account for some of the variability in focus inhibition reported in the literature.

Topics: Amphotericin B; Animals; Cell Line; Cell Transformation, Neoplastic; Drug Synergism; Hydrogen-Ion Concentration; Mice; Moloney murine leukemia virus; Rifamycins; Viral Plaque Assay

Topics: Amphotericin B; Animals; Animals, Newborn; Antineoplastic Agents; Carbon Isotopes; Cell Survival; Cell Transformation, Neoplastic; Cycloheximide; Dactinomycin; Drug Synergism; Fibroblasts; Fusidic Acid; In Vitro Techniques; Leucine; Lung; Microscopy, Electron; Phenylalanine; Polysaccharides; Protein Biosynthesis; Rats; Tritium; Uracil