amoxicillin-potassium-clavulanate-combination and Rectal-Neoplasms

We report the first case of Gordonibacter pamelaeae bacteremia, identified by phenotypic tests and 16S rRNA sequencing in a patient with disseminated rectosigmoid carcinoma and responsive to amoxicillin-clavulanate. The bacterium was a nonsporulating, anaerobic, gram-positive, nonmotile, coccobacillus that was catalase, arginine dihydrolase, and arginine acrylamidase positive. The gastrointestinal tract is probably its reservoir.

Topics: Actinobacteria; Aged, 80 and over; Amoxicillin-Potassium Clavulanate Combination; Anti-Bacterial Agents; Bacteremia; Bacterial Typing Techniques; DNA, Bacterial; DNA, Ribosomal; Gram-Positive Bacterial Infections; Humans; Male; Molecular Sequence Data; Rectal Neoplasms; RNA, Ribosomal, 16S; Sequence Analysis, DNA

A gram-negative alkaline phosphatase- and pyrrolidone peptidase-positive rod-shaped bacterium (CCUG 45702) was isolated from two aerobic blood cultures from a female cancer patient. No identification could be reached using phenotypic techniques. Amplification of the tRNA intergenic spacers revealed fragments with lengths of 116, 133, and 270 bp, but no such pattern was present in our reference library. Sequencing of the 16S rRNA gene revealed its identity as Moraxella atlantae, a species isolated only rarely and published only once as causing infection. In retrospect, the phenotypic characteristics fit the identification as M. atlantae (formerly known as CDC group M-3). Comparative 16S rRNA sequence analysis indicates that M. atlantae, M. lincolnii, and M. osloensis might constitute three separate genera within the MORAXELLACEAE: After treatment with amoxicillin-clavulanic acid for 2 days, fever subsided and the patient was dismissed.

Topics: Adenocarcinoma; Adult; Amoxicillin-Potassium Clavulanate Combination; Bacteremia; Base Sequence; DNA, Bacterial; DNA, Ribosomal; Female; Genes, Bacterial; Humans; Molecular Sequence Data; Moraxella; Neisseriaceae Infections; Opportunistic Infections; Rectal Neoplasms; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Homology, Nucleic Acid; Species Specificity