amanitins and Leukemia--Monocytic--Acute

amanitins has been researched along with Leukemia--Monocytic--Acute* in 1 studies

Other Studies

1 other study(ies) available for amanitins and Leukemia--Monocytic--Acute

Article	Year
Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1. Biochemical and biophysical research communications, 1988, Oct-31, Volume: 156, Issue:2 The acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level of mRNA as IL-1 beta mRNA is more abundant than IL-1 alpha mRNA. Studies of transcription in isolated nuclei however indicate that each gene is transcribed at a similar rate, suggesting that post-transcriptional mechanisms regulate the relative abundance of IL-1 alpha and IL-1 beta mRNA. Measurement of RNA half life after addition of alpha-amanitin (an inhibitor of RNA polymerase II) indicate that IL-1 alpha mRNA is not as stable as IL-1 beta mRNA suggesting one mechanism for the different relative levels of RNA. Topics: Amanitins; Cycloheximide; Female; Gene Expression Regulation; Half-Life; Humans; Interleukin-1; Kinetics; Leukemia, Monocytic, Acute; Lipopolysaccharides; RNA Polymerase II; RNA, Messenger; Tetradecanoylphorbol Acetate; Transcription, Genetic; Tumor Cells, Cultured	1988

Article

Year

Post-transcriptional control of IL-1 gene expression in the acute monocytic leukemia line THP-1.

Biochemical and biophysical research communications, 1988, Oct-31, Volume: 156, Issue:2

The acute monocytic leukemia cell line THP-1 secretes predominantly IL-1 beta after treatment with bacterial lipopolysaccharide and tumour promoting phorbol ester (PMA). IL-1 alpha is also secreted, but represents less than 10% of the total IL-1 activity. This differential is reflected at the level of mRNA as IL-1 beta mRNA is more abundant than IL-1 alpha mRNA. Studies of transcription in isolated nuclei however indicate that each gene is transcribed at a similar rate, suggesting that post-transcriptional mechanisms regulate the relative abundance of IL-1 alpha and IL-1 beta mRNA. Measurement of RNA half life after addition of alpha-amanitin (an inhibitor of RNA polymerase II) indicate that IL-1 alpha mRNA is not as stable as IL-1 beta mRNA suggesting one mechanism for the different relative levels of RNA.

Topics: Amanitins; Cycloheximide; Female; Gene Expression Regulation; Half-Life; Humans; Interleukin-1; Kinetics; Leukemia, Monocytic, Acute; Lipopolysaccharides; RNA Polymerase II; RNA, Messenger; Tetradecanoylphorbol Acetate; Transcription, Genetic; Tumor Cells, Cultured

1988