amanitins and Carcinoma--Krebs-2

The incubation of Krebs ascites tumor cells in medium with a high salt concentration resulted in a partial inhibition of nuclear RNA synthesis. The residual RNA polymerase activity in such nuclei was only slightly inhibited by low concentrations (50 nM) of alpha-amanitin. This finding suggested an inhibition of RNA polymerase II activity under conditions of medium hypertonicity. Indeed, RNA polymerase II, isolated from the nuclei of cells exposed to hypertonicity, revealed only half of the control activity. On the other hand, RNA polymerase I was not affected by hypertonicity. Moreover, chromatin fractions isolated from cells incubated in hypertonic or isotonic medium were equally template-active in RNA synthesis.

Topics: Amanitins; Animals; Carcinoma, Krebs 2; Cell Nucleus; Cycloheximide; Hypertonic Solutions; Mice; Mice, Inbred BALB C; RNA; RNA Polymerase II; Sodium Chloride