amanitins and Body-Weight

In 3-methylcholanthrene (3-MC)-pretreated young rats the free and engaged RNA polymerases were investigated in detail both in the nucleolar and nucleoplasmic fractions. Both functional forms of the nucleolar RNA polymerase (A) show a great stimulation. The maximum increase of the free form up to 235% compared with controls is obtained 24 h after 3-MC administration. The engaged fraction reaches its maximum later. The extranucleolar RNA polymerase (B) in its engaged form shows the maximum effect (160%) after 24 h, whereas the free form is at first diminished and later on increases steadily. The activation of RNA polymerase A and the interconversion process between the free and engaged form of RNA polymerase B seem to play a significant role in the 3-MC-mediated induction.

Topics: Amanitins; Animals; Body Weight; Cell Nucleus; DNA-Directed RNA Polymerases; Enzyme Induction; Injections, Intraperitoneal; Liver; Methylcholanthrene; Rats

The effectiveness of three plasma cleansing techniques (exchange transfusion, peritoneal dialysis and plasmapheresis) is studied in the treatment of phalloides intoxication. The severity of the latter is dependent upon the amount of toxin ingested, with can now be measured by radio-immunology, as well as the fixation of the toxin in the liver. Methods to ensure its elimination must be instituted as soon as possible. Thus in 43 patients who had consumed more than 50 g of fresh fungi, there were no deaths amongst those treated during the first 36 hours and 7 deaths out of 22 patients treated late. It is not yet possible to define the respective values of each method.

Topics: Adolescent; Adult; Agaricales; Aged; Amanita; Amanitins; Blood Transfusion; Body Weight; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Mushroom Poisoning; Oligopeptides; Peritoneal Dialysis; Phalloidine; Radioimmunoassay; Time Factors

Topics: Amanitins; Animals; Animals, Newborn; Body Weight; Brain; Cell Nucleus; Dactinomycin; DNA; DNA-Directed RNA Polymerases; Female; Hypothyroidism; Kinetics; Liver; Magnesium; Male; Manganese; Organ Size; Organ Specificity; Osmolar Concentration; Rats; Thyroidectomy; Transcription, Genetic