amanitins and Acute-Phase-Reaction

Acute phase responses to intragastric administration of a single dose of CCl(4) were examined with albumin mRNA turnover as a marker. Based on the combination of the changes in stability of albumin mRNA and activity of transcription of its gene, the entire course of liver injury was classified into three stages, the first stage for aggravation of injury until 9 h, the second from 9 to 24 h, and the third for repair of injury or regeneration of liver after 48 h. Liver S100 fractions from normal and CCl(4)-treated rats contained, in total, 11 polypeptides cross-linked with part of albumin mRNA, although they did not appear to be specific to this mRNA. Their profiles were altered together with the changes in stability of albumin mRNA in different stages. These findings suggest that the polypeptides with distinct properties play roles in physiologically significant processes involved in utilization and turnover of albumin mRNA, apparently altering its stability.

Topics: Acute-Phase Reaction; Albumins; Amanitins; Animals; Base Sequence; Binding, Competitive; Carbon Tetrachloride; Chemical and Drug Induced Liver Injury; Gene Expression Regulation; Half-Life; Liver; Liver Diseases; Male; Molecular Weight; Peptides; Protein Binding; Rats; Rats, Wistar; RNA Probes; RNA Stability; RNA-Binding Proteins; RNA, Messenger; Substrate Specificity; Time Factors; Transcription, Genetic