alvocidib and Atherosclerosis

Atherosclerotic coronary artery disease (CAD) is one of the most prevalent diseases worldwide. Atherosclerosis was considered to be the single most important contributor to CAD. In this study, a distinct serum protein expression pattern in CAD patients was demonstrated by proteomic analysis with two-dimensional gel electrophoresis coupled with mass spectrometry. In particular, CDK9 was found to be highly elevated in serum, monocytes and artery plaque samples of CAD patients. Furthermore, there was high infiltration of CD14+ monocytes/macrophages within artery plaques correlated with the expression of CDK9. Moreover, Flavopiridol (CDK9 inhibitor) could inhibit THP-1 cell (monocytic acute leukemia cell line) proliferation by targeting CDK9. Altogether, These findings indicate that CDK9 represent an important role for inflammation in the pathogenesis of atherosclerosis. It may be a potential biomarker of atherosclerotic inflammation and offer insights into the pathophysiology and targeted therapy for atherosclerotic CAD.

Topics: Aged; Apoptosis; Atherosclerosis; Biomarkers; Cell Cycle; Cell Line, Tumor; Cell Proliferation; Cells, Cultured; Coronary Artery Disease; Cyclin-Dependent Kinase 9; Female; Flavonoids; Gene Expression; Humans; Immunohistochemistry; Inflammation; Male; Middle Aged; Monocytes; Piperidines; Protein Kinase Inhibitors; Proteomics; Reverse Transcriptase Polymerase Chain Reaction