aluminum-tetrasulfophthalocyanine and Uterine-Cervical-Neoplasms

Background Material and Methods Results Conclusions.

Topics: 1,2-Dipalmitoylphosphatidylcholine; Carcinoma, Squamous Cell; Cell Line, Tumor; Female; Head and Neck Neoplasms; HeLa Cells; Humans; Indoles; Isoindoles; Liposomes; Mouth Neoplasms; Organometallic Compounds; Photochemotherapy; Photosensitizing Agents; Squamous Cell Carcinoma of Head and Neck; Uterine Cervical Neoplasms; Zinc Compounds

The effects of Photodynamic Therapy using 2nd generation photosensitizers have been widely investigated aiming clinical application treatment of solid neoplasms. In this work, ultrastructure changes caused by the action of two 2nd generation photosensitizers and laser irradiation on CHO-K1 and HeLa (neoplastic) cells were analyzed by transmission electron microscopy. Aluminum phthalocyanine chloride, aluminum phthalocyanine tetrasulfonate chloride and radiation from a semiconductor laser at a fluency of 0.5 J/cm2 (Power=26 mW; lambda=.670 nm) were used. The results showed induction of apoptosis. Such alterations where observed in HeLa but not in CHO-K1 cells after Aluminum phthalocyanine tetrasulfonate chloride (AlPcS4, photodynamic treatment. The Aluminum phthalocyanine chloride (AlPc) photodynamic treatment induced necrosis on the neoplastic cell line, and cytoplasm and nuclear alterations on the normal cell line.

Topics: Animals; Apoptosis; Cell Nucleus; CHO Cells; Cricetinae; Cytoplasm; Female; HeLa Cells; Humans; Indoles; Lasers; Light; Microscopy, Electron; Mitochondria; Necrosis; Organometallic Compounds; Ovary; Photic Stimulation; Photochemotherapy; Radiation-Sensitizing Agents; Uterine Cervical Neoplasms

This work relates to studies on modes of phototoxicity by tetrasulfonated aluminium phthalocyanine (AlPcS4), tetrahydroxy- and monosulfonated meso-tetraphenylporphines (3-THPP and TPPS1) on culture cells. Toxicity at moderate light exposures appears to be related to inhibition of microtubule function. Treatment of human cervix carcinoma cells of the line NHIK 3025 incubated for 18 h with the sensitizers and exposed to light inhibits multiplication for the first hours after light exposure, a significant fraction of the cells accumulating in mitosis. For the first hours after treatment, the mitotic cells were always mainly found in metaphase; generally seen as c-metaphases and three-group metaphases. During this time, anaphase and telophase cells were absent or greatly reduced in number. Indirect immunofluorescence staining of beta-tubulin showed that the spindle apparatus of mitotic cells was perturbed in all cases. The accumulation in mitosis was more extensive after treatment with AlPcS4 and light than after treatment with 3-THPP or TPPS1 and light. This may be related to the great difference in the lipophilic properties of these sensitizers; i.e. AlPcS4 being highly water soluble while TPPS1 and 3-THPP are lipophilic sensitizers. The lipophilicity of several sensitizers has been measured by two different methods, the partition between an aqueous and a lipophilic phase (Triton X-114) and the binding strength to a reverse phase column. The results show that the measured relative lipophilicity of the sensitizers may be influenced by the method of analysis.

Topics: Carcinoma in Situ; Cell Division; Cell Line; Female; Humans; Indoles; Light; Mitosis; Organometallic Compounds; Radiation-Sensitizing Agents; Uterine Cervical Neoplasms