aluminum-tetrasulfophthalocyanine and Inflammation

Hypoxia is a major contributor to global kidney diseases. Targeting hypoxia is a promising therapeutic option against both acute kidney injury and chronic kidney disease; however, an effective strategy that can achieve simultaneous targeted kidney hypoxia imaging and therapy has yet to be established. Herein, we fabricated a unique nano-sized hypoxia-sensitive coassembly (Pc/C5A@EVs) via molecular recognition and self-assembly, which is composed of the macrocyclic amphiphile C5A, the commercial dye sulfonated aluminum phthalocyanine (Pc) and mesenchymal stem cell-excreted extracellular vesicles (MSC-EVs).. This synergetic nanoscale coassembly with great translational potential provides a novel strategy for precise kidney hypoxia diagnosis and efficient kidney injury treatment. Furthermore, our strategy of coassembling exogenous macrocyclic receptors with endogenous cell-derived membranous structures may offer a functional platform to address multiple clinical needs.

Topics: Acute Kidney Injury; Animals; Calixarenes; Cell Hypoxia; Cell Line; Epithelial Cells; Extracellular Vesicles; Humans; Hypoxia-Inducible Factor 1, alpha Subunit; Indoles; Inflammation; Integrins; Macrocyclic Compounds; Macrophages; Mice; NF-kappa B; Organometallic Compounds; Signal Transduction; Surface-Active Agents

The aim of this study was evaluating changes in the photosensitizer fluorescence in vivo in the radiation-induced damage area in comparison of intact areas with a simultaneous assessment of changes both in blood parameters and in histological data.. The study was conducted in white outbred SHK mice (n = 21). Their right hindlimbs were irradiated with a dose of 25 Gy after the intraperitoneal injections of photosensitizer 'Photosens'. Fluorescence intensity was traced in vivo by a laser diagnostic system for seven weeks. Simultaneously, histological examination of the damaged areas and blood tests were performed.. An increased intensity of the laser-induced fluorescence of the photosensitizer 'Photosens' in the damaged areas, compared to the intact symmetrical ones was observed. Laboratory blood tests and histological examination showed changes that may indicate the occurrence of inflammation.. Enhanced intensity of the exogenous fluorescence of the photosensitizer in the radiation-induced inflammation of noncancerous tissues was observed. The obtained results may potentially affect an interpretation of the results of intraoperative tumors navigation that have been previously irradiated and can be used for selection of an individualized dose fractionation algorithm in radiology.

Topics: Algorithms; Animals; Body Weight; Dose-Response Relationship, Radiation; Female; Fluorescence; Indoles; Inflammation; Lasers; Mice; Organometallic Compounds; Photosensitizing Agents; Radiation Injuries, Experimental; Time Factors; X-Rays