alpinumisoflavone and Osteoporosis

alpinumisoflavone has been researched along with Osteoporosis* in 2 studies

Other Studies

2 other study(ies) available for alpinumisoflavone and Osteoporosis

ArticleYear
Alpinumisoflavone rescues glucocorticoid-induced apoptosis of osteocytes via suppressing Nox2-dependent ROS generation.
    Pharmacological reports : PR, 2018, Volume: 70, Issue:2

    Long term use of glucocorticoids is one of the most common causes of secondary osteoporosis. Osteocyte, the most abundant cell type in bone, coordinates the function of osteoblast and osteoclast. This study evaluates the protective effect of alpinumisoflavone (AIF), a naturally occurring flavonoid compound, on dexamethasone (Dex)-induced apoptosis of osteocytes.. MLO-Y4 cell was used as a cell model. The effect of AIF on the cell viability was assessed by MTT assay. Apoptosis of MYL-Y4 cells was determined by DNA fragment detection ELISA kit and flow cytometry. Intracellular ROS level was determined by DCFH-DA staining. mRNA and protein expression of target genes were determined by qRT-PCR and western blot, respectively.. AIF effectively protected MLO-Y4 cells against Dex-induced apoptosis, which was associated with attenuation of Dex-induced ROS generation in MLO-Y4 cells. Furthermore, our data indicated that the expression of NAD(P)H oxidase 2 (Nox2) was suppressed by AIF, which in turn mediated the attenuating effect on Dex-induced ROS generation and apoptosis in MLO-Y4 cells. Moreover, our results showed that AIF modulated the expression of Nox2 by activating AMPK signaling.. AIF activated AMPK-dependent Nox2 signaling pathway to suppress Dex-induced ROS production in cultured osteocytes, which might explain its anti-apoptotic effect. These results indicate that activation of AMPK pathway by AIF could have beneficial effects on bone damage induced by excessive oxidative stress and osteocyte apoptosis.

    Topics: AMP-Activated Protein Kinase Kinases; Animals; Apoptosis; Cell Line; Dexamethasone; Glucocorticoids; Isoflavones; Mice; NADPH Oxidase 2; Osteoblasts; Osteoclasts; Osteocytes; Osteoporosis; Protein Kinases; Reactive Oxygen Species; Signal Transduction

2018
Alpinumisoflavone protects against glucocorticoid-induced osteoporosis through suppressing the apoptosis of osteoblastic and osteocytic cells.
    Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2017, Volume: 96

    The long-term use of glucocorticoids is found to cause osteoporosis. This study is designed to evaluate the protective effect of alpinumisoflavone (AIF), a naturally occurring flavonoid compound, on dexamethasone(Dex)-induced osteoporosis. We use a rat model to investigate the apoptosis of osteoblastic and osteocytic cells. The results indicate that AIF effectively protects against dexamethasone-induced osteoporosis. Moreover, AIF effectively reversed dexamethasone-induced apoptosis in osteoblastic and osteocytic cells through inhibiting ROS overproduction and regulating the Nrf2 pathway. In conclusion, the AIF activated Nrf2 signaling pathway was observed to suppress Dex-induced ROS production in osteoblastic and osteocytic cells, which may explain its anti-osteoporotic effects against dexamethasone-induced osteoporosis.

    Topics: 3T3 Cells; Animals; Apoptosis; Cell Line; Dexamethasone; Glucocorticoids; Isoflavones; Male; Mice; NF-E2-Related Factor 2; Osteoblasts; Osteoporosis; Protective Agents; Rats; Rats, Sprague-Dawley; Reactive Oxygen Species; Signal Transduction

2017