alpha-synuclein and Craniofacial-Abnormalities

Evaluation of motor deficits in rodents is mostly restricted to limb motor tests that are often high stressors for the animals.. To test rodents for orofacial motor impairments in a stress-free environment, we established the pasta gnawing test by measuring the biting noise of mice that eat a piece of spaghetti. Two parameters were evaluated, the biting speed and the biting peaks per biting episode. To evaluate the power of this test compared to commonly used limb motor and muscle strength tests, three mouse models of Parkinson's disease, amyotrophic lateral sclerosis and Niemann-Pick disease were tested in the pasta gnawing test, RotaRod and wire suspension test.. Our results show that the pasta gnawing test reliably displays orofacial motor deficits.. The test is especially useful as additional motor test in early onset disease models, since it shows first deficits later than the RotaRod or wire suspension test. The test depends on a voluntary eating behavior of the animal with only a short-time food deprivation and should thus be stress-free.. The pasta gnawing test represents a valuable tool to analyze orofacial motor deficits in different early onset disease models.

Topics: alpha-Synuclein; Amyotrophic Lateral Sclerosis; Animals; Craniofacial Abnormalities; Disease Models, Animal; DNA-Binding Proteins; Exercise Test; Humans; Intracellular Signaling Peptides and Proteins; Mastication; Mice; Mice, Transgenic; Motor Activity; Muscle Strength; Niemann-Pick C1 Protein; Niemann-Pick Diseases; Parkinson Disease; Proteins; Psychomotor Performance; Reaction Time; Rotarod Performance Test; Statistics, Nonparametric