alpha-cyperone and Lung-Diseases

Avian pathogenic Escherichia coli (APEC) are extra-intestinal pathogenic E. coli, and usually cause avian septicemia through breaching the blood-gas barrier. Type II pneumocytes play an important role of maintaining the function of the blood-gas barrier. However, the mechanism of APEC injuring type II pneumocytes remains unclear. α-cyperone can inhibit lung cell injury induced by Staphylococcus aureus. In order to explore whether α-cyperone regulates the adherence and invasion of APEC-O78 to chicken type II pneumocytes, we successfully cultured chicken type II pneumocytes. The results showed that α-cyperone significantly decreased the adherence of APEC-O78 to chicken type II pneumocytes. In addition, α-cyperone inhibited actin cytoskeleton polymerization induced by APEC-O78 through down regulating the expression of Nck-2, Cdc42 and Rac1. These results provide new evidence for the prevention of colibacillosis in chicken.

Topics: Alveolar Epithelial Cells; Animals; Bacterial Adhesion; Cell Survival; Chickens; Escherichia coli; Escherichia coli Infections; Humans; Lung Diseases; Naphthalenes; Poultry Diseases; Reverse Transcriptase Polymerase Chain Reaction; RNA, Bacterial; Specific Pathogen-Free Organisms