alpha-chymotrypsin and Staphylococcal-Infections

The experience with treatment of 104 patients with non-specific pleural empyema without bronchial fistula is summarized. The local use of antibacterial agents in combination with proteolytic enzymes against the background of rational general immunocorrective therapy permitted to cure without operative intervention 124 patients. Four patients were operated on. They underwent decortication of a lung with pleurectomy. One patient died.

Topics: Acute Disease; Anti-Bacterial Agents; Chymotrypsin; Combined Modality Therapy; Drainage; Empyema; Escherichia coli Infections; Humans; Pseudomonas Infections; Staphylococcal Infections; Trypsin

20 patients with chronic staphylococcal osteomyelitis were tested for 6 acute phase reactants: alpha 1-antitrypsin, orosomucoid, haptoglobin, ceruloplasmin, C-reactive protein (CRP) and antichymotrypsin during different phases of the disease. CRP was best correlated to clinical activity and in 3 cases CRP and ceruloplasmin increased a few weeks before a clinically apparent exacerbation of the osteomyelitis took place. A Latex-CRP slide method showed fairly good agreement with CRP assessed by immunoassay. Determination of CRP is a suitable test for following the activity of chronic osteomyelitis.

Topics: alpha 1-Antichymotrypsin; alpha 1-Antitrypsin; Alpha-Globulins; C-Reactive Protein; Ceruloplasmin; Chronic Disease; Chymotrypsin; Haptoglobins; Humans; Immunoassay; Latex; Male; Orosomucoid; Osteomyelitis; Prospective Studies; Staphylococcal Infections; Trypsin Inhibitors

The effect of lincomycin, chymotrypsin and their combination on the blood count of the plaque-autoforming cells (PAFC) and immune adhesion (IA) of staphylococci was studied on mice with staphylococcal infection. The infected mice were divided into 4 groups: nontreated, lincomycin treated, chymotrypsin treated, and lincomycin + chymotrypsin treated. The doses of lincomycin and chymotrypsin were 150 and 2 mg/kg, respectively. The mice were decapitated by the 3rd, 7th, 14th or 21st day of the infection and treatment and the blood was collected for the count of the PAFC and IA. A decrease in the rate of IA with a simultaneous increase in the PAFC count was found in the untreated mice and the mice treated with lincomycin alone. The use of chymotrypsin and its combination with lincomycin stimulated IA and lowered the level of autosensibilization.

Topics: Animals; Chymotrypsin; Drug Therapy, Combination; Hemolytic Plaque Technique; Immune Adherence Reaction; Lincomycin; Mice; Staphylococcal Infections; Staphylococcus aureus

The effect of lincomycin, chymotrypsin and their combinations on the plasmocytic reaction of the spleen and agglutinin titer in mice with experimental staphylococcal infection was studied. The infected mice were divided into 4 groups: the 1st group included untreated infected animals, the 2nd, 3rd and 4th groups consisted of the mice treated with lincomycin, chymotrypsin and their combinations respectively. Lincomycin and chymotrypsin were used in doses of 150 and 2 mg/kg respectively. By the 3rd, 7th, 14th and 21st day of the infection and treatment the animals were decapitated, th blood was collected for determination of the staphylococcal agglutinin titers in the serum, the spleens were removed for investigation of the plasmocytic reaction. It was shown that the treatment of the experimental staphylococcal infection with lincomycin resulted in decreased proliferation of the plasmatic cells and antibody formation. The use of chymotrypsin resulted in increased proliferation of the plasmatic cells and specific antibody titer. The use of chymotrypsin in conjunction with lincomycin lowered the suppressing effect of the latter on the above indices.

Topics: Animals; Antibodies, Bacterial; Chymotrypsin; Drug Evaluation, Preclinical; Lincomycin; Mice; Plasma Cells; Spleen; Staphylococcal Infections; Staphylococcus; Time Factors

The culture of Staphylococcus aureus was administered intraperitoneally in a dose of LD30 to albino mice. The animals of the 1st, 2nd and 3rd groups were treated with lincomycin, chymotripsin and combination of lincomycin with chymotripsin respectively. The animals of the 4th group were used as control and were not subjected to the treatment with the drugs. A part of the animals from every group was killed on the 3rd, 7th, 14th, 21st and subsequent days and their organs were investigated microscopically and bacteriologically. It was found that staphylococci was isolated from the control mice during a 50-day period after inoculation. Complete liberation of the organs from the causative agent within 25 days from the beginning of the experiment was registered in the animals treated with lincomycin. Isolation of the staphylococci was over by the 27th day in the animals treated with chymotrypsin. Liberation of the organs from the causative agent by the 17th day was observed in the albino mice treated with the combination of lincomycin with chymotrypsin. The combined use of lincomycin with chymotrypsin proved to be most effective: no death was registered among the albino mice, the levels of the pathogenicity and antibiotic resistance in the pathogenic staphylococci decreased.

Topics: Animals; Chymotrypsin; Drug Combinations; Drug Evaluation, Preclinical; Lincomycin; Mice; Staphylococcal Infections; Time Factors

The effect of leucomycin, chimotrypsin and their combination on the leucocyte phagocytic activity was studied on mice with experimental staphylococcal infection. Lincomycin and chimotrypsin were administered in doses of 150 and 2 mg/kg respectively. In the study of the leucocyte phagocytic activity it was found on the 3rd, 7th, 14th and 21st days after the beginning of the animal infection and treatment that the experimental staphylococcal infection stimulated the non-specific phagocytosis. Lincomycin inhibited the leucocyte phagocytic activity. The use of chimotrypsin in the process of the staphylococcal infection treatment resulted in increased phagocytosis activity. The combined use of chimotrypsin and leucomycin decreased the inhibitory effect of the latter on phagocytosis.

Topics: Animals; Chymotrypsin; Drug Combinations; Drug Evaluation, Preclinical; Leukocytes; Lincomycin; Mice; Phagocytosis; Staphylococcal Infections; Time Factors

Topics: Adult; Anabolic Agents; Child; Chymotrypsin; Decanoates; Humans; Nandrolone; Pyrimidines; Staphylococcal Infections; Wound Infection

Topics: Animals; Chymotrypsin; Humans; Inflammation; Rabbits; Staphylococcal Infections

Topics: Animals; Chlorophyll; Chymotrypsin; Drug Therapy, Combination; Eucalyptus; Female; Plant Extracts; Plants, Medicinal; Rabbits; Salpingitis; Staphylococcal Infections

Topics: Anti-Bacterial Agents; Antibiotics, Antitubercular; Antitussive Agents; Bronchitis; Bronchitis, Chronic; Chloramphenicol; Chymotrypsin; Haemophilus influenzae; Humans; Penicillins; Pneumococcal Infections; Staphylococcal Infections; Streptomycin; Trypsin