alpha-chymotrypsin and Skin-Neoplasms

The aim of this work is to evaluate the antitumor effect mediated by the proteasome inhibitors of

Topics: 9,10-Dimethyl-1,2-benzanthracene; Animals; Antineoplastic Agents; Caco-2 Cells; Chymotrypsin; Drug Evaluation, Preclinical; Female; Humans; Inula; Mice; Molecular Docking Simulation; Papilloma; Plant Extracts; Proteasome Endopeptidase Complex; Proteasome Inhibitors; Skin Neoplasms; Toxicity Tests

The effect of combined proteolytic enzymes, administered by the rectal route, on the metastatic process and the time of survival in C57Bl6 mice with the Lewis lung carcinoma inoculated subcutaneously was investigated. In the control group, which received no enzyme treatment, 90% of animals died of the metastatic spread of cancer by day 18 after primary tumor extirpation. In Group A, which received the multi-enzyme solution from the time of primary tumor extirpation, 30% of mice died of disseminated cancer by day 25. In Group B, which was treated with the enzymes from 6 days before primary tumor extirpation, only 10% of animals showed the metastatic process by day 15. In Group C, which received the enzymes from 24 hours after intracutaneous tumor inoculation, no metastatic dissemination was discernible. In these three groups, the enzyme treatment was carried out throughout the study. None of the control animals survived for 100 days when the study was ended. The treated groups A, B and C showed survival rate 60%, 90% and 100% of animals, respectively, by 100 days.

Topics: Administration, Rectal; Animals; Carcinoma, Lewis Lung; Chymotrypsin; Drug Combinations; Endopeptidases; Female; Mice; Mice, Inbred C57BL; Neoplasm Metastasis; Neoplasm Transplantation; Pancreatic Extracts; Papain; Skin Neoplasms; Survival Rate; Thymus Extracts; Trypsin

Mast cell populations can be distinguished by differences in the content and substrate specificity of their two major cytoplasmic granule proteases, the chymases and the tryptases. To explore the origins of differences in the types of proteases present in mast cells, we used a double cytochemical staining technique to reveal both chymase and tryptase in cells from four lines of dog mast cell tumors containing both enzymes. We expected that if chymase and tryptase were expressed together during cell development the relative staining intensity of chymase compared to tryptase would be constant among different cells of each tumor. Instead, we found substantial variation in the relative intensity of chymase and tryptase staining among cells of a given mastocytoma line, each of which contained cells presumed to be monoclonal in origin but heterogeneous with respect to cell development. The overall staining intensity for chymase or tryptase correlated with the amount of protease activity in extracts of tumor homogenates. Staining specificity was established by use of selective inhibitors and competitive substrates and was tested on various types of dog cells obtained by bronchoalveolar lavage. The results suggest that active chymase and tryptase may be expressed differently during mast cell differentiation and support the possibility of a close developmental relationship between mast cells differing in protease phenotype. Moreover, the success of the staining procedures applied to mastocytoma cells suggests that they may be of general utility in phenotyping of mast cells according to the protease activities present in their granules.

Topics: Animals; Bronchoalveolar Lavage Fluid; Chymases; Chymotrypsin; Dogs; Histocytochemistry; Mast Cells; Mast-Cell Sarcoma; Peptide Hydrolases; Serine Endopeptidases; Skin Neoplasms; Trypsin; Tumor Cells, Cultured

Topics: Animals; Carcinogens; Cell Transformation, Neoplastic; Chymotrypsin; DNA Damage; Glycine max; Humans; Mice; Pancreatic Neoplasms; Protease Inhibitors; Skin Neoplasms; Superoxides; Trypsin Inhibitors

A case of chronic monocytic leukemia with specific dermal lesions is presented. The patient developed an adenocarcinoma of the cecum.

Topics: Aged; alpha 1-Antichymotrypsin; Chymotrypsin; Female; Humans; Immunoenzyme Techniques; Leukemia, Myeloid; Microscopy, Electron; Monocytes; Skin; Skin Neoplasms

Forty-one cases of dermatofibrosarcoma protuberans are presented. The clinical features and histopathological appearances are described. Immunohistochemical staining of thirteen cases with antisera to lysozyme, alpha 1-antichymotrypsin and S-100 protein has provided no evidence to support either a histiocytic or neuroectodermal origin for these tumours. In reviewing the literature, the histogenetic origin, differential diagnosis and malignant potential of dermatofibrosarcoma protuberans are discussed.

Topics: Adolescent; Adult; Aged; alpha 1-Antichymotrypsin; Chymotrypsin; Female; Fibrosarcoma; Humans; Male; Middle Aged; Muramidase; S100 Proteins; Skin Neoplasms

Topics: alpha 1-Antichymotrypsin; alpha 1-Antitrypsin; Chymotrypsin; Fibroma; Humans; Skin Neoplasms; Staining and Labeling

Epidermal mononuclear cell infiltrate from three patients with pagetoid reticulosis was examined for the presence of the cytoplasmic markers lysozyme, alpha 1-antitrypsin and alpha 1-antichymotrypsin, using specific antisera and a peroxidase-antiperoxidase technique. Many of the infiltrating cells possessed these markers, indicating that they belonged to the monocyte-macrophage-histiocyte series.

Topics: Adult; alpha 1-Antitrypsin; Chymotrypsin; Histiocytes; Histocytochemistry; Humans; Lymphatic Diseases; Macrophages; Male; Middle Aged; Monocytes; Muramidase; Skin Neoplasms

Topics: Animals; BCG Vaccine; Carcinoma, Hepatocellular; Cell Wall; Chymotrypsin; Hydrochloric Acid; Injections; Liver Neoplasms; Lymphatic Metastasis; Male; Mice; Mycobacterium; Mycobacterium bovis; Mycobacterium tuberculosis; Neoplasm Transplantation; Neoplasms, Experimental; Nitrosamines; Nocardia asteroides; Pronase; Propionibacterium acnes; Skin Neoplasms; Sodium Hydroxide; Transplantation, Homologous; Trypsin

Topics: Animals; Benz(a)Anthracenes; Cell Division; Cell Nucleus; Cell Transformation, Neoplastic; Chymotrypsin; DNA; Epithelial Cells; Epithelium; Fluorometry; Hyperplasia; Male; Methods; Mice; Mitosis; Neoplasms, Experimental; Precancerous Conditions; Skin; Skin Neoplasms

Three synthetic inhibitors of proteases (tosyl lysine chloromethyl ketone, tosyl phenylalanine chloromethyl ketone, and tosyl arginine methyl ester) inhibit the tumorigenesis initiated in mouse skin by 7,12-dimethylbenz(a)anthracene and promoted by croton oil or its active principle, phorbol ester. These protease inhibitors, when applied directly to mouse skin, inhibit some of the irritant effects of the tumor promoter and are not toxic.

Topics: Animals; Antineoplastic Agents; Arginine; Benz(a)Anthracenes; Cell Transformation, Neoplastic; Chymotrypsin; Croton Oil; Depression, Chemical; Esters; Ketones; Lysine; Mice; Papain; Phenylalanine; Skin Neoplasms; Terpenes; Trypsin Inhibitors