alpha-chymotrypsin and Sarcoma

Topics: Amylases; Animals; Arginase; Benz(a)Anthracenes; Carcinoma; Carcinoma 256, Walker; Carcinoma, Brown-Pearce; Carcinoma, Ehrlich Tumor; Catalase; Chymotrypsin; Deoxyribonucleases; Enzyme Therapy; Humans; Hyaluronoglucosaminidase; Mammary Neoplasms, Experimental; Mice; Neoplasms, Experimental; Osteosarcoma; Peroxidases; Rats; Ribonucleases; Sarcoma; Sarcoma 180; Sarcoma, Experimental; Trypsin

Using an avidin-biotin immunoperoxidase technic, the authors studied 29 anaplastic thyroid tumors (ATTs) to determine the frequency of hormonal (thyroglobulin--TG; calcitonin--CT), epithelial (epithelial membrane antigen, monoclonal keratin), or sarcoma (desmin; alpha-1-antichymotrypsin--ACT; vimentin) markers. Their results indicate that 27% of ATTs stain for TG and none for CT. Fifty-five percent showed epithelial markers, 48% marked for ACT, and 47% for vimentin. Coexpression of keratin and vimentin was found in 39% of cases tested. The expression of the tested antigens did not correlate significantly with histologic pattern (epithelial vs. "sarcomatous"). Of note is the fact that 30% of the ATTs the authors tested expressed none of the markers they examined, indicating total lack of differentiation.

Topics: Adult; Aged; Aged, 80 and over; Anaplasia; Calcitonin; Carcinoma; Chymotrypsin; Epithelium; Female; Humans; Immunoenzyme Techniques; Keratins; Male; Membrane Proteins; Middle Aged; Mucin-1; Sarcoma; Thyroglobulin; Thyroid Neoplasms; Vimentin

Fourteen cases of mixed müllerian tumour (MMT) of the endometrium, six stromal sarcomas, five uterine leiomyosarcomas, and five leiomyomas were examined, using an immunoperoxidase technique, for the presence of alpha-1-antitrypsin (A1AT), alpha-1-antichymotrypsin (A1ACT), actin, and myosin. In addition, six cases of endometrial adenocarcinoma were examined for the presence of A1AT and A1ACT. Eleven MMT were positive for A1AT and 13 for A1ACT. All stromal sarcomas, four adenocarcinomas, four leiomyomas, and three leiomyosarcomas were positive for both. All leiomyomas and leiomyosarcomas were positive for myosin as were seven. MMT and three stromal sarcomas. Four leiomyomas, four leiomyosarcomas, six MMT, and three stromal sarcomas were positive for actin. These findings are related to those in the normal uterus and their practical and theoretical significance discussed.

Topics: Actins; Adenocarcinoma; alpha 1-Antichymotrypsin; alpha 1-Antitrypsin; Chymotrypsin; Endometriosis; Female; Histocytochemistry; Humans; Leiomyoma; Leiomyosarcoma; Myosins; Sarcoma; Uterine Neoplasms

Topics: Animals; Carbon Radioisotopes; Cell Line; Cell Transformation, Neoplastic; Centrifugation, Density Gradient; Chymotrypsin; Electrophoresis, Polyacrylamide Gel; Fibroblasts; Gammaretrovirus; Glucosamine; Glycoproteins; Iodine Radioisotopes; Leucine; Molecular Weight; Moloney murine leukemia virus; Peroxidases; Rats; Rats, Inbred Strains; Sarcoma; Tritium; Trypsin; Viral Proteins

Topics: Animals; Antibody Specificity; Antigens; Antigens, Neoplasm; Autolysis; Chemistry Techniques, Analytical; Chymotrypsin; Edetic Acid; Epitopes; Histocompatibility; Hydrogen-Ion Concentration; Immune Sera; Methods; Mice; Papain; Peptide Hydrolases; Sarcoma; Solubility; Spleen; Thermolysin; Transplantation Immunology; Trypsin