alpha-chymotrypsin and Necrosis

Topics: Aged; alpha 1-Antichymotrypsin; Chymotrypsin; Collagen; Female; Histiocytes; Histiocytoma, Benign Fibrous; Humans; Mitosis; Muramidase; Necrosis; Stomach; Stomach Neoplasms; Vimentin

This study aimed to evaluate the clinical therapeutic efficacy of anti-snake venom serum blockade in treating local tissue necrosis caused by Chinese cobra (Naja atra) bites.. Patients bitten by a Chinese cobra (Naja atra) (n = 50) that met the inclusion criteria were randomly divided into two groups: the experimental group (n = 25) and the control group (n = 25). The experimental group received regular as well as anti-snake venom serum blocking treatment, whereas regular treatment plus chymotrypsin blocking therapy was given to the control group. The necrotic volumes around snake wounds in these groups were detected on the first, third and seventh days. On the third day of treatment, some local tissues in the wounds were randomly selected for pathological biopsy, and the necrosis volume of the local tissue was observed. Furthermore, the amount of time required for wound healing was recorded.. On the third and seventh days post-treatment, the necrotic volume of the wound of the experimental group was much smaller than that of the control group, and the experimental group's wound healing time was shorter than that of the control group (all p < 0.05). Moreover, the pathological biopsies taken from the control group showed nuclear pyknosis, fragmentation, sparse nuclear density, and blurred edges, and the degree of necrosis was much higher than that of the experimental group.. Anti-snake venom blocking therapy is a new and improved therapy with good clinical effect on local tissue necrosis caused by Chinese cobra bites; moreover, it is superior to conventional chymotrypsin blocking therapy in the treatment of cobra bites. It can better neutralize and prevent the spread of the toxin, reduce tissue necrosis, and shorten the course of the disease by promoting healing of the wound. Furthermore, this treatment plan is also applicable to wound necrosis caused by other snake toxins, such as tissue necrosis caused by elapidae and viper families.. This trial is registered in the Chinese Clinical Trial Registry, a primary registry of International Clinical Trial Registry Platform, World Health Organization (Registration No. ChiCTR2200059070; trial URL:http://www.chictr.org.cn/edit.aspx?pid=134353&htm=4).

Topics: Animals; Antivenins; Chymotrypsin; Elapid Venoms; Elapidae; Humans; Naja naja; Necrosis; Snake Bites

A comparative study was performed of the caseinolytic, necrolytic and fibrinolytic activities of a crab collagenase product prepared from the hepatopancreas of the king crab (Paralithodes camtschatica) and four enzyme preparations (trypsin/chymotrypsin and protease complexes isolated from Aspergillus terricola, Carica papaya and pseudomonodaceae). This paper reports an in vitro investigation, an in vivo study with rats and a clinical evaluation. It was found that crab collagenase has the highest proteolytic activity measured with respect to fibrin clot and necrotic eschar in vitro although its caseinolytic activity is the lowest. A clinical trial involving 21 patients with leg ulcers confirmed the clinical efficiency of crab collagenase compared with trypsin/ chymotrypsin. The results suggest that crab collagenase is useful in wound debridement.

Topics: Aged; Animals; Brachyura; Chymotrypsin; Collagenases; Debridement; Drug Evaluation, Preclinical; Endopeptidases; Female; Humans; Leg Ulcer; Male; Middle Aged; Necrosis; Rats; Rats, Wistar; Trypsin; Wound Healing

Topics: Acute Disease; Aged; Chymotrypsin; Female; Follow-Up Studies; Humans; Male; Necrosis; Pancreatectomy; Pancreatitis; Radiography; Trypsin

Topics: Animals; Chymotrypsin; Dogs; Intestinal Diseases; Necrosis; Shock, Hemorrhagic; Trypsin Inhibitors

Topics: Animals; Bone Diseases; Chymotrypsin; Dogs; Necrosis; Periosteum; Shoulder; Time Factors; Wound Healing

Topics: Adolescent; Adult; Chymotrypsin; Colectomy; Colitis, Ulcerative; Colon; Colonic Neoplasms; Female; Gastrointestinal Hemorrhage; Humans; Ileostomy; Intestinal Mucosa; Intestinal Polyps; Male; Middle Aged; Necrosis; Protease Inhibitors; Secretory Rate; Trypsin; Trypsin Inhibitors

Topics: Acute Disease; Animals; Caseins; Chymotrypsin; Dogs; Electrophoresis; Necrosis; Pancreas; Pancreatitis; Peptide Hydrolases; Tissue Extracts; Trypsin; Trypsin Inhibitors

Topics: Adult; Aged; Celiac Artery; Chronic Disease; Chymotrypsin; Cysts; Diagnosis, Differential; Hemoperitoneum; Humans; Male; Middle Aged; Necrosis; Pancreatic Elastase; Pancreatitis; Radiography; Spleen; Splenic Artery; Splenic Diseases; Splenic Rupture

Topics: Animals; Carotid Arteries; Cats; Chymotrypsin; Collagen Diseases; Dura Mater; Endopeptidases; Inflammation; Injections, Spinal; Muscles; Necrosis; Nerve Degeneration; Subarachnoid Space

Established centers find that cytological study of gastric washings with saline or chymotrypsin, adequately performed, is a valuable diagnostic tool in the detection of early and curable gastric carcinoma.Our experience with a small series of 150 patients, studied by saline gastric washing, has emphasized the difficulties of collection and the particular importance of obtaining, by repeated washings if necessary, an adequate specimen of gastric epithelial cells for diagnosis, before an opinion is given. It seems likely that the cytological method will be of future value in study of the natural history of gastric malignant disease and in detection of its surface lesions in their earliest form in asymptomatic, known-susceptible persons. Further, it should become a complementary part of the "stomach profile" in gastric diagnostic problems, where roentgenologic and gastroscopic studies may be expected to reveal the older, necrotic, or infiltrative lesions; cytological study, the earlier and more superficial stages of disease.

Topics: Cell Count; Chymotrypsin; Epithelial Cells; Gastroscopy; Humans; Necrosis; Sodium Chloride; Stomach Neoplasms

Topics: Abscess; Chymotrypsin; Debridement; Endopeptidases; Humans; Hydrolases; Necrosis; Peptide Hydrolases