alpha-chymotrypsin has been researched along with Mouth-Diseases* in 16 studies
16 other study(ies) available for alpha-chymotrypsin and Mouth-Diseases
Article | Year |
---|---|
Immune response and alveolar bone resorption in a mouse model of Treponema denticola infection.
Treponema denticola is considered to be an agent strongly associated with periodontal disease. The lack of an animal infection model has hampered the understanding of T. denticola pathogenesis and the host's immune response to infection. In this study, we have established an oral infection model in mice, demonstrating that infection by oral inoculation is feasible. The presence of T. denticola in the oral cavities of the animals was confirmed by PCR. Mice given T. denticola developed a specific immune response to the bacterium. The antibodies generated from the infection were mainly of the immunoglobulin G1 subclass, indicating a Th2-tilted response. The antibodies recognized 11 T. denticola proteins, of which a 62-kDa and a 53-kDa protein were deemed immunodominant. The two proteins were identified, respectively, as dentilisin and the major outer sheath protein by mass spectrometry. Splenocytes cultured from the infected mice no longer produced interleukin-10 and produced markedly reduced levels of gamma interferon relative to those produced by naïve splenocytes upon stimulation with T. denticola. Mandibles of infected mice showed significantly greater bone resorption (P < 0.01) than those of mock-infected controls. Topics: Alveolar Bone Loss; Amino Acid Sequence; Animals; Antibodies, Bacterial; Bacterial Proteins; Chymotrypsin; Disease Models, Animal; Female; Mice; Mice, Inbred C57BL; Mouth Diseases; Peptide Hydrolases; Porins; Treponema denticola; Treponemal Infections | 2009 |
Control of bacterial infection for effective treatment of oral mucositis.
Topics: Chymotrypsin; Drug Therapy, Combination; Fibroblast Growth Factor 7; Fibroblast Growth Factors; Humans; Mouth Diseases; Mouth Mucosa; Papain; Trypsin | 2002 |
Patterns of immunoreactivity to an anti-fibronectin polyclonal antibody in formalin-fixed, paraffin-embedded oral tissues are dependent on methods of antigen retrieval.
The influence of antigen retrieval (AR) technique on immunoreactivity in formalin-fixed, paraffin-embedded tissues is recognized. In focal reactive overgrowths of oral mucosa, we noted that patterns of immunoreactivity to a fibronectin polyclonal antibody were dependent on methods of AR. To establish these patterns we investigated eight pyogenic granulomas and eight fibroepithelial polyps. In the absence of AR no immunoreactivity was observed. After alpha-chymotrypsin AR a band of intense immunoreactivity was associated with vascular endothelial cells, with either minimal or no staining of connective tissue. After microwave AR immunoreactivity was observed in connective tissue, especially in the subepithelial region, but there was no specific vascular staining. After autoclave AR immunoreactivity was observed in connective tissue and also in epithelial nuclei. To determine if these results represented either changes induced by tissue fixation and processing or exposure of epitopes normally masked in vivo, we investigated frozen sections from two fibroepithelial polyps and one pyogenic granuloma. In frozen sections immunoreactivity was observed around vascular endothelial cells and within connective tissue, especially in the subepithelial region, but not in epithelia. This study provides indirect evidence that alpha-chymotrypsin and heat-mediated AR protocols expose different masked epitopes, and reinforces the need to undertake appropriate pilot studies for immunohistochemical investigation of archival tissue. Topics: Antibodies; Antigens; Chymotrypsin; Connective Tissue; Endothelium, Vascular; Fibronectins; Formaldehyde; Granuloma, Pyogenic; Humans; Immunohistochemistry; Indicators and Reagents; Mouth Diseases; Paraffin; Polyps; Retrospective Studies; Staining and Labeling | 1995 |
[Clinical study of the new Dontisolon M].
Topics: Anti-Infective Agents; Chymotrypsin; Drug Combinations; Endopeptidases; Mouth Diseases; Neomycin; Prednisolone; Trypsin; Urea | 1988 |
Oral submucous fibrosis--a new treatment regimen.
Successful treatment of oral submucous fibrosis with local injections of chymotrypsin, hyaluronidase, and dexamethasone is reported. In resistant cases, surgical excision of the fibrotic bands with submucosal placement of fresh human placental grafts was found to be successful. Topics: Chymotrypsin; Combined Modality Therapy; Dexamethasone; Humans; Hyaluronoglucosaminidase; Mouth Diseases; Oral Submucous Fibrosis; Placenta; Placental Extracts; Random Allocation | 1988 |
[Leukoedema of the oral mucosa. Anatomo-clinical review. Some observations].
Topics: Adolescent; Adult; Biopsy; Cholagogues and Choleretics; Chymotrypsin; Female; Humans; Leukoedema, Oral; Male; Microscopy; Middle Aged; Mouth Diseases; Mouth Mucosa; Tongue; Vitamin B Complex | 1973 |
[Enzymatic-antibiotic combination in odontology. 1st observations].
Topics: Anti-Inflammatory Agents; Chymotrypsin; Humans; Infections; Mouth Diseases; Postoperative Complications; Tetracycline; Trypsin | 1970 |
[Use of proteolytic enzymes in the treatment of acute suppurative diseases of the maxillo-facial area].
Topics: Acute Disease; Cellulitis; Chymotrypsin; Focal Infection, Dental; Humans; Jaw Diseases; Mouth Diseases; Osteomyelitis; Peptide Hydrolases; Trypsin | 1969 |
[Advantages of a tetracycline-alphachymotrypsin combination in dental practice].
Topics: Chymotrypsin; Dentistry; Drug Synergism; Mouth Diseases; Tetracycline | 1968 |
["Dontisolon-oral healing paste" as adhesive dressing].
Topics: Administration, Topical; Anti-Inflammatory Agents; Chymotrypsin; Humans; Mouth Diseases; Neomycin; Occlusive Dressings; Ointments; Prednisolone; Stomatitis; Trypsin | 1968 |
[Clinical evaluation of the effect of a combination of tetracycline-L-methylenbysine with trypsin and alpha-chymotrypsin on acute and chronic oral infections].
Topics: Chymotrypsin; Dentistry; Drug Synergism; Mouth Diseases; Tetracycline; Trypsin | 1968 |
[The use of an antibiotic-enzyme combination in odontostomatological practice].
Topics: Anti-Infective Agents; Anti-Inflammatory Agents; Chymotrypsin; Drug Synergism; Humans; Inflammation; Mouth Diseases; Tetracycline; Trypsin | 1968 |
[Clinical experimentation with oral administration of a combination of trypsin, chymotrypsin and tetracycline in odontostomatology].
Topics: Anti-Infective Agents; Anti-Inflammatory Agents; Chymotrypsin; Drug Synergism; Humans; Inflammation; Mouth Diseases; Tetracycline; Trypsin | 1968 |
[Inflammation and anti-inflammatory agents. Clinical experiences with non-steroid anti-inflammatory drugs in oral surgery].
Topics: Anti-Inflammatory Agents; Bromelains; Chymotrypsin; Humans; Indomethacin; Inflammation; Mouth Diseases; Oxyphenbutazone; Streptokinase | 1968 |
[Therapeutic study of anti-inflammatory enzymatic synergism].
Topics: Anti-Inflammatory Agents; Chymotrypsin; Drug Synergism; Humans; Mouth Diseases; Trypsin | 1967 |
[Experimental trial of a new injectable proteolytic enzyme in stomatologic practice].
Topics: Chymotrypsin; Glycoside Hydrolases; Humans; Injections; Mouth Diseases | 1966 |