alpha-chymotrypsin and Hypoglycemia

Topics: Administration, Oral; Adsorption; Animals; Azo Compounds; Blood Glucose; Chemical Phenomena; Chemistry; Chymotrypsin; Hydrolysis; Hypoglycemia; Insulin; Intubation, Gastrointestinal; Male; Naphthalenes; Pepsin A; Polystyrenes; Rabbits

Proinsulin in nearly homogeneous form has been isolated from a preparation of porcine insulin. A molecular weight close to 9100 was calculated from the amino acid composition and from sedimentation-equilibrium studies. Through the action of trypsin this single-chain protein is transformed to desalanine insulin by cleavage of a polypeptide chain connecting the carboxy-terminus of the B chain to the amino-terminus of the A chain of insulin. The amino acid sequence of this connecting peptide was found to be Arg-Arg-Glu-Ala-Gln-Asn-Pro-Gln-Ala-Gly-Ala-Val-Glu-Leu-Gly-Gly-Gly-Leu-Gly-Gly-Leu-Gln-Ala-Leu-Ala-Leu-Glu-Gly-Pro-Pro-Gln-Lys-Arg.

Topics: Amino Acid Sequence; Animals; Biological Assay; Cellulose; Chromatography, Ion Exchange; Chymotrypsin; Electrophoresis, Disc; Hypoglycemia; Insulin; Molecular Weight; Peptides; Proteins; Seizures; Swine; Trypsin

Topics: Alloxan; Animals; Chymotrypsin; Diabetes Mellitus, Experimental; Histidine; Hypoglycemia; Pharmacology; Rats; Research; Serine; Trypsin

Topics: Animals; Blood Glucose; Carbohydrate Metabolism; Chymotrypsin; Diaphragm; Dogs; Epididymis; Glucose; Humans; Hypoglycemia; In Vitro Techniques; Insulin; Male; Rats; Toxicology; Trypsin