alpha-chymotrypsin has been researched along with Gastroenteritis--Transmissible--of-Swine* in 1 studies
1 other study(ies) available for alpha-chymotrypsin and Gastroenteritis--Transmissible--of-Swine
Article | Year |
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A double-protease-resistant variant of transmissible gastroenteritis virus and its ability to induce lactogenic immunity.
A virus resistant to 2 major intestinal proteases (trypsin and alpha-chymotrypsin) was derived from the attenuated Purdue strain of transmissible gastroenteritis virus. Its enzymatic stability was confirmed, in vitro, by exposure to proteolytic enzymes and to porcine intestinal fluids. Vaccination of 5 seronegative pregnant sows with the variant virus by a series of 2 oral and 1 IM inoculations resulted in high titers of neutralizing antibody in serum and colostrum. The mean antibody titer in milk whey decreased 44-fold within 1 week after parturition. At 3 days of age, the 40 pigs delivered by these sows were challenge exposed orally with virulent transmissible gastroenteritis virus. Pigs nursing the 5 vaccinated sows underwent a relatively mild clinical course of illness. The average mortality of these 40 pigs was 33%. Thirty-six pigs which had been raised by 4 nonvaccinated sows had a more severe illness, greater daily weight loss, and higher mortality (92%). Topics: Animals; Animals, Suckling; Antibodies, Viral; Chymotrypsin; Colostrum; Coronaviridae; Drug Resistance, Microbial; Female; Gastroenteritis, Transmissible, of Swine; Genetic Variation; Milk; Neutralization Tests; Pregnancy; Swine; Transmissible gastroenteritis virus; Trypsin; Vaccination; Vaccines, Attenuated; Viral Vaccines | 1985 |