alpha-chymotrypsin and Arthritis

Topics: Arthritis; Back Pain; Chymotrypsin; Clinical Trials as Topic; Humans; Placebos; Spinal Diseases; Trypsin

Rheumatoid arthritis (RA) is mainly characterized by synovial hyperplasia, angiogenesis, inflammatory cells infiltration. Chymotrypsin is a proteolytic enzyme with anti-inflammatory effects. The current project was intended to test the efficacy and mechanism of chymotrypsin in adjuvant-induced arthritis (AIA) rats to provide an experimental basis for the clinical application of chymotrypsin.. The body weight of the rats in each group showed an increasing trend, and there was no significant difference in weight between the groups. CHD and piroxicam suppressed paw swelling and arthritic scores and decreased synovial hyperplasia, inflammatory cell infiltration, pannus formation, and bone destruction. Furthermore, the overproduction of MMP-1, TNF-α, IL-1β, and IL-6 in serum was remarkably attenuated in the chymotrypsin- and piroxicam-treated rats. The protein levels of TLR4 and NF-κB in the synovial tissue of the chymotrypsin group and the piroxicam group were significantly lower than those in the model group. Likewise, the rats treated with chymotrypsin and piroxicam had a substantial decline in the mRNA expression of TLR4, NF-κB, TNF-α, IL-1β, and IL-6 in synovial tissue.. Chymotrypsin alleviates the joint damage of AIA rats, probably by reducing the expression of MMP-1, TNF-α, IL-1β, and IL-6 through TLR4/NF-κB signaling pathway.

Topics: Animals; Arthritis; Body Weight; Chymotrypsin; Hyperplasia; Interleukin-6; Matrix Metalloproteinase 1; NF-kappa B; Rats; Rats, Sprague-Dawley; RNA, Messenger; Toll-Like Receptor 4; Tumor Necrosis Factor-alpha

Topics: Animals; Antigens; Arthritis; Cell Movement; Chromatography, Gel; Chymotrypsin; Collagen; Female; Hot Temperature; Humans; Isoflurophate; Lymphokines; Neuraminidase; Neutrophils; Puromycin; Rats; Rats, Inbred Strains

Extracts of human peripheral blood polymorphonuclear leukocyte granules, and two purified proteases derived from such extracts, an elastase and a chymotrypsin-like enzyme, degrade isolated bovine nasal cartilage proteoglycan at neutral pH. Viscosity studies indicate that the leukocyte granule extracts lack hyaluronidase activity and that their degradative effect on proteoglycan at physiological pH is due entirely to proteolytic action. Sepharose 4B gel chromatography and SDS-polyacrylamide gel electrophoresis of proteoglycan fractions treated with leukocyte granule enzymes at pH 7.0 indicate that they degrade one of the proteoglycan link proteins, release a fragment from the hyaluronic acid-binding portion of the proteoglycan subunit core protein, and break down the remainder of the proteoglycan subunit molecule into peptide fragments with varying numbers of chondroitin sulfate chains. Immunodiffusion studies indicate that the antigenic determinants of the proteoglycan subunit core protein and the link proteins survive treatment with granule proteases. Similar degradation of human articular cartilage proteoglycan by granule neutral proteases can be presumed to occur, in view of the similarity of structure of human articular and bovine nasal cartilage proteoglycans. The release of granule enzymes in the course of neutrophil-mediated inflammation can thus result in the degradation of cartilage matrix proteoglycan, leading to cartilage destruction and joint injury.

Topics: Animals; Arthritis; Cartilage, Articular; Cattle; Chemical Phenomena; Chemistry; Chondroitin Sulfates; Chromatography, Gel; Chymotrypsin; Cytoplasmic Granules; Disease Models, Animal; Electrophoresis, Polyacrylamide Gel; Glycosaminoglycans; Humans; Immunodiffusion; Joint Diseases; Leukocytes; Nasal Septum; Pancreatic Elastase; Proteoglycans; Sodium Dodecyl Sulfate; Viscosity

Topics: Acetates; Animals; Anti-Inflammatory Agents; Antifungal Agents; Arthritis; Carrageenan; Chymotrypsin; Dextrans; Edema; Fatty Acids; Formaldehyde; Hindlimb; Irritants; Kaolin; Lactones; Male; Mustard Plant; Plants, Medicinal; Rats; Yeasts

Topics: Arthritis; Arthritis, Rheumatoid; Chymotrypsin; Cyclophosphamide; Femoral Artery; Heparin; Humans; Injections, Intra-Articular; Lupus Erythematosus, Systemic; Scleroderma, Systemic; Spondylitis, Ankylosing

Topics: Anti-Bacterial Agents; Arthritis; Arthritis, Rheumatoid; Cataract; Cataract Extraction; Chymotrypsin; Diabetes Mellitus; Drug Therapy; Endophthalmitis; Eye Diseases; Eye Foreign Bodies; Humans; Lens, Crystalline; Ophthalmia, Sympathetic; Panophthalmitis; Postoperative Complications; Steroids; Uveitis; Vitreous Body

Topics: Anti-Inflammatory Agents; Arthritis; Body Temperature; Burns; Chymotrypsin; Infrared Rays; Oxyphenbutazone; Photography; Skin; Sprains and Strains; Thermography; Wound Healing; Wounds and Injuries

Topics: Anti-Bacterial Agents; Arthritis; Arthritis, Infectious; Arthritis, Rheumatoid; Chymotrypsin; Humans; Trypsin

Topics: Arthritis; Arthritis, Rheumatoid; Chymotrypsin; Humans; Proteins