alpha-(4-pyridyl-1-oxide)-n-tert-butylnitrone and Leukemia--Myeloid

alpha-(4-pyridyl-1-oxide)-n-tert-butylnitrone has been researched along with Leukemia--Myeloid* in 1 studies

Other Studies

1 other study(ies) available for alpha-(4-pyridyl-1-oxide)-n-tert-butylnitrone and Leukemia--Myeloid

Article	Year
Detection of lipid radicals by electron paramagnetic resonance spin trapping using intact cells enriched with polyunsaturated fatty acid. The Journal of biological chemistry, 1992, Mar-25, Volume: 267, Issue:9 Electron paramagnetic resonance (EPR) spin trapping was used to detect lipid-derived free radicals generated by iron-induced oxidative stress in intact cells. Using the spin trap alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), carbon-centered radical adducts were detected. These lipid-derived free radicals were formed during incubation of ferrous iron with U937 cells that were enriched with docosahexaenoic acid (22:6n-3). The EPR spectra exhibited apparent hyperfine splittings characteristic of a POBN/alkyl radical, aN = 15.63 +/- 0.06 G and aH = 2.66 +/- 0.03 G, generated as a result of beta-scission of alkoxyl radicals. Spin adduct formation depended on the FeSO4 content of the incubation medium and the number of 22:6-enriched cells present; when the cells were enriched with oleic acid (18:1n-9), spin adducts were not detected. This is the first direct demonstration, using EPR, of a lipid-derived radical formed in intact cells in response to oxidant stress. Topics: Cell Line; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Fatty Acids, Unsaturated; Free Radicals; Humans; Leukemia, Myeloid; Nitrogen Oxides; Pyridines; Spin Labels	1992

Article

Year

Detection of lipid radicals by electron paramagnetic resonance spin trapping using intact cells enriched with polyunsaturated fatty acid.

The Journal of biological chemistry, 1992, Mar-25, Volume: 267, Issue:9

Electron paramagnetic resonance (EPR) spin trapping was used to detect lipid-derived free radicals generated by iron-induced oxidative stress in intact cells. Using the spin trap alpha-(4-pyridyl 1-oxide)-N-tert-butylnitrone (POBN), carbon-centered radical adducts were detected. These lipid-derived free radicals were formed during incubation of ferrous iron with U937 cells that were enriched with docosahexaenoic acid (22:6n-3). The EPR spectra exhibited apparent hyperfine splittings characteristic of a POBN/alkyl radical, aN = 15.63 +/- 0.06 G and aH = 2.66 +/- 0.03 G, generated as a result of beta-scission of alkoxyl radicals. Spin adduct formation depended on the FeSO4 content of the incubation medium and the number of 22:6-enriched cells present; when the cells were enriched with oleic acid (18:1n-9), spin adducts were not detected. This is the first direct demonstration, using EPR, of a lipid-derived radical formed in intact cells in response to oxidant stress.

Topics: Cell Line; Cyclic N-Oxides; Electron Spin Resonance Spectroscopy; Fatty Acids, Unsaturated; Free Radicals; Humans; Leukemia, Myeloid; Nitrogen Oxides; Pyridines; Spin Labels

1992