allopurinol and Polycystic-Ovary-Syndrome

The aim of this study is to examine women with polycystic ovary syndrome (PCOS) to determine the relationship between xanthine oxidase (XO) and oxidative stress, inflammatory status, and various clinical and biochemical parameters. In this cross-sectional study a total of 83 women including 45 PCOS patients and 38 healthy women were enrolled. We collected blood samples for XO and superoxide dismutase (SOD) activity, hormone levels, cholesterol values, and inflammatory markers. Body mass index (BMI) , waist-to-hip ratio (WHR), and blood pressure were assessed. Blood samples were taken for hormonal levels, cholesterol levels, fasting plasma glucose (FPG), fasting plasma insulin (FPI), homeostatic model assessment-insulin resistance (HOMA-IR) index, quantitative insulin sensitivity check index (QUICKI), C-reactive protein (CRP), white blood cell and neutrophil counts, XO and SOD activities. The basal hormone levels, triglyceride (TG) levels, TG/HDL-C (high density lipoprotein-cholesterol) ratios FPG, FPI and HOMA-IR levels were higher in PCOS patients compared to controls (p<0.05). Platelet and plateletcrit (PCT) values, CRP, and XO activity were significantly increased, however SOD activity was decreased in PCOS patients (p<0.001). XO activity was positively correlated with LH/FSH and TG/HDL ratios, CRP, PCT, FPG, FPI, and HOMA-IR, and negatively correlated with QUICKI levels. In conclusion, XO is a useful marker to assess oxidative stress in PCOS patients. Positive correlations between XO and inflammatory markers and cardiovascular disease risk factors suggest that XO plays an important role in the pathogenesis of PCOS and its metabolic complications.

Topics: Adult; Biomarkers; Blood Glucose; C-Reactive Protein; Cardiovascular Diseases; Cholesterol; Cross-Sectional Studies; Female; Hormones; Humans; Inflammation; Insulin; Oxidative Stress; Polycystic Ovary Syndrome; Prognosis; Risk; Superoxide Dismutase; Triglycerides; Xanthine Oxidase; Young Adult

To investigate whether there is any relation between oxidative stress and the antioxidant system in the development of polycystic ovary syndrome (PCOS) by measuring serum nitric oxide (NO) levels and xanthine oxidase (XO) activity (a generator of reactive oxygen species) and antioxidant status by measuring serum thiol levels and glutathione peroxidase (GSHPx) and paraoxonase 1 (PON1) activities.. Prospective case-control study.. University hospital in Turkey.. Thirty women with polycystic ovary syndrome and 20 age- and sex-matched healthy control subjects were included.. Serum XO, PON1 and GSHPx activity and NO and thiol levels were determined by spectrophotometric methods.. Activity of serum XO, PON1 and GSH, as well as NO and thiol levels.. Serum XO activities were higher in women with PCOS than in the control women (p<0.001). The PON1 activity was lower in women with PCOS than in the control women (p<0.001). No significant difference was found between NO and thiol levels and GSHPx activities of women with PCOS and the control women (p>0.05). Serum PON1 activities were negatively correlated with serum XO activities and NO levels.. Increased oxidant XO activity and decreased lipid antioxidant PON1 activity, along with the observed negative correlation between these parameters, suggests that women with PCOS are under oxidative stress and that there is XO-mediated lipid peroxidation, which may be related to increased atherosclerosis seen in later life in such women.

Topics: Adult; Antioxidants; Aryldialkylphosphatase; Biomarkers; Case-Control Studies; Female; Glutathione Peroxidase; Humans; Lipid Peroxidation; Nitric Oxide; Oxidative Stress; Polycystic Ovary Syndrome; Prospective Studies; Spectrophotometry; Sulfhydryl Compounds; Xanthine Oxidase