allopurinol and Mastitis--Bovine

Characterization of milk fat globule (MFG) was performed to investigate the difference in MFG membrane (MFGM) between fresh and mastitis Holstein Friesian cow milk. Lipid distribution investigated by exogenous phospholipids using microscopy showed higher phospholipid content in fresh compared to mastitic MFGM. Xanthine oxidase assay indicative of membrane impairment revealed lower activity in mastitic samples compared to fresh globules. Of note, significantly higher roughness of globule surface and zeta potential was observed in mastitis compared to fresh globules. Influence of globule membrane on the interaction with L. fermentum demonstrated preferential adhesion of bacteria to fresh compared to mastitic globules including enhanced extent of binding. Results of the present study provides an insight of the interfacial changes occurring at the globule surface as well as highlighting the importance of selective bacterial interaction with milk components for the potential development of functional food with relevance to human health.

Topics: Animals; Cattle; Female; Flow Cytometry; Food Analysis; Glycolipids; Glycoproteins; Lipid Droplets; Mastitis, Bovine; Microscopy, Atomic Force; Phospholipids; Structure-Activity Relationship; Xanthine Oxidase

The effect of mastitis on the milk fat globule membrane of milk samples from individual quarters was investigated. Wisconsin Mastitis Test positive (greater than 20mm) milk contained approximately 10% less milk fat globule membrane material than negative (smaller than 10mm) milk. The lipid portion of the milk fat globule membrane preparation from positive milk contained smaller amounts of phospholipids and larger amounts of cholesterol. Milk fat globule membrane preparations from positive milk had higher aldolase activity and lower xanthine oxidase activity. Dissociated membrane protein prepared from positive milk was resolved into five components by polyacrylamide gel electrophoresis while the membrane protein prepared from negative milk was resolved into three components.

Topics: Animals; Cattle; Cholesterol; Electrophoresis, Polyacrylamide Gel; Fats; Female; Fructose-Bisphosphate Aldolase; Lipase; Lipids; Mastitis, Bovine; Methods; Milk; Milk Proteins; Phospholipids; Xanthine Oxidase

Topics: Aniline Compounds; Animals; Catalase; Cattle; Female; Glucosephosphate Dehydrogenase; Mastitis, Bovine; Milk; NAD; Oxidoreductases; Peroxidases; Phosphogluconate Dehydrogenase; Xanthine Oxidase