allopurinol and Exfoliation-Syndrome

The aim of this study was to determine serum and aqueous xanthine oxidase (XO) levels, and mRNA expression in anterior lens epithelial cells in pseudoexfoliation (PEX).. In this prospective study, serum, aqueous and anterior lens capsules were taken from 21 patients with PEX and 23 normal subjects who had undergone routine cataract surgery. Serum and aqueous XO levels were analyzed using the colorimetric method. mRNA expression of XO in anterior lens epithelial cells was evaluated using reverse transcription polymerase chain reaction analysis.. Serum XO levels (means ± standard deviations) were 207.0 ± 86.1 IU/mL and 240.6 ± 114.1 IU/mL in the normal and PEX groups, respectively (p = 0.310). Aqueous XO levels (means ± standard deviations) were 65.5 ± 54.3 IU/mL in the normal group and 130.5 ± 117.4 IU/mL in the PEX group (p = 0.028). There was a 2.9 fold decrease in mRNA expression in anterior lens epithelial cells of PEX, which is significantly lower than the normal group (p = 0.01).. Higher aqueous XO levels lacking associated different serum XO suggests higher oxidative stress in the aqueous. Higher aqueous XO levels in PEX with decreased mRNA expression in anterior lens epithelial cells indicate possible overexpression of XO in other structures related to the aqueous.

Topics: Aged; Aged, 80 and over; Anterior Capsule of the Lens; Aqueous Humor; Epithelial Cells; Exfoliation Syndrome; Female; Gene Expression Regulation, Enzymologic; Humans; Intraocular Pressure; Male; Prospective Studies; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger; Visual Acuity; Xanthine Oxidase

Our purpose was to investigate the possible roles of nitrite levels and the activity of paraoxonase (PON), xanthine oxidase (XO) and adenosine deaminase (ADA) in the pathogenesis of pseudoexfoliation (PEX) syndrome.. Serum samples were taken from 43 patients with PEX and 41 control subjects. The serum PON, XO and ADA activities and nitrite levels were assayed by spectrophotometric methods.. The PON, ADA and XO activities and nitrite levels (means +/- standard deviation) were 81.72 +/- 42.56 U/ml, 19.75 +/- 5.72 U/l, 0.16 +/- 0.09 U/l and 94.23 +/- 27.72 micromol/l in the PEX group, respectively. In the control group, the PON, ADA and XO activities and the nitrite levels were 111.02 +/- 43.51 U/ml, 15.21 +/- 3.93 U/l, 0.13 +/- 0.06 U/l and 61.96 +/- 19.05 micromol/l, respectively. The serum PON activity was significantly lower in the PEX group when compared with the control group. The serum XO and ADA activities and nitrite levels were significantly higher in the patients with PEX when compared with the control subjects.. Decreased PON (an antioxidant enzyme) activity as well as increased ADA and XO activities and nitrite levels indicate that oxidative stress is increased and purine metabolism is altered in PEX syndrome.

Topics: Adenosine Deaminase; Aged; Aryldialkylphosphatase; Exfoliation Syndrome; Humans; Middle Aged; Nitric Oxide; Nitrites; Oxidative Stress; Purines; Spectrophotometry; Xanthine Oxidase