alisol-b-monoacetate and Reperfusion-Injury

The ligand-activated nuclear receptor, farnesoid X receptor (FXR), plays a pivotal role in regulating renal function. Activation of FXR by its specific agonists exerts renoprotective action in animals with acute kidney injury (AKI). In the present study, we aimed to identify naturally occurring agonists of FXR with potential as therapeutic agents in renal ischemia-reperfusion injury. In vitro and in vivo FXR activation was determined by a dual-luciferase assay, docking analysis, site-directed mutagenesis, and whole kidney transcriptome analysis. Wild-type (WT) and FXR knockout (

Topics: Acute Kidney Injury; Animals; Apoptosis; Cholestenones; Disease Models, Animal; Drugs, Chinese Herbal; HEK293 Cells; Hep G2 Cells; Humans; Inflammation Mediators; Kidney; Ligands; Male; Mice, Inbred C57BL; Mice, Knockout; Oxidative Stress; Receptors, Cytoplasmic and Nuclear; Reperfusion Injury; Signal Transduction