aldrin and Carcinoma--Hepatocellular

Five established hepatoma cell lines, 1 of rat origin and 4 derived from human liver carcinoma, were compared for their capacity to perform metabolic activation of one polycyclic hydrocarbon, benzo[a]pyrene (BP), and one cyclodiene chlorinated insecticide, aldrin. The results of these investigations indicated both species and individual differences among these cell lines. Aldrin was found to be converted into dieldrin much more efficiently by the rat hepatoma cell line than by any human cell lines, whereas 2 human lines displayed the highest BP-metabolizing activity whether measured as the amounts of water-soluble products or estimated by cytotoxic cell-mediated assays. Results also showed that one particular human cell line can replace, advantageously, V79 hamster cells as target in a cell-mediated assay owing to its incapability to metabolize BP and its high sensitivity to BP metabolites.

Topics: Aldrin; Animals; Benzo(a)pyrene; Benzopyrenes; Biotransformation; Carcinogens; Carcinoma, Hepatocellular; Cell Line; Cell Survival; Cricetinae; Cricetulus; Cytotoxicity, Immunologic; Humans; Kinetics; Liver Neoplasms; Liver Neoplasms, Experimental; Lung; Rats; Species Specificity

Topics: Aldrin; Animals; Benzopyrene Hydroxylase; Carcinoma, Hepatocellular; Cell Differentiation; Cell Line; Cholesterol 7-alpha-Hydroxylase; Cytochrome P-450 Enzyme System; Enzyme Induction; Epoxy Compounds; Liver Neoplasms; Mixed Function Oxygenases; Oxygenases; Rats; Steroid 12-alpha-Hydroxylase; Steroid Hydroxylases; Substrate Specificity

The development of hyperplastic and neoplastic lesions of parenchymal cells of the liver adjacent to central veins was observed in C3H mice ingesting the chlorinated hydrocarbons, dieldrin or aldrin, in the diet. Lesions could be followed from pericentral hyperplasia to areas of hyperplasia, nodules of hyperplasia, small hepatocellular carcinomas, and large well-developed carcinomas, occasionally with metastases. Sometimes pericentral hyperplasia was diffuse throughout most or all of one lobe of the liver. These hyperplastic cells collided to become one large nodule and also one large carcinoma. The carcinomas were well-differentiated or moderately well-differentiated and grew on transplantation to isologous hosts. Histologically, the hyperplastic cells adjacent to central veins were increased in size, frequently with double nuclei. Carcinoma cells varied in size and shape and were huge with large nuclei, prominent nucleoli, and eosinophilic cytoplasm. Similar hepatocellular carcinomas were seen previously with carbon tetrachloride, another organochlorine chemical.

Topics: Aldrin; Animals; Carcinoma, Hepatocellular; Chemical and Drug Induced Liver Injury; Dieldrin; Female; Hepatic Veins; Hyperplasia; Liver Diseases; Liver Neoplasms; Male; Mice; Mice, Inbred C3H