alcian-blue and Respiratory-Syncytial-Virus-Infections

Respiratory syncytial virus-induced bronchiolitis has been linked to the development of allergy and atopic asthma. IL-12 and possibly IL-18 are central mediators orchestrating Th1 and/or Th2 immune responses to infection. To determine a possible role for IL-12 in regulating the immune response to acute respiratory syncytial virus infection, IL-12p40 gene-targeted (IL-12p40-/-) and wild-type mice were intratracheally infected with respiratory syncytial virus, and lung inflammatory and immune responses were assessed. Lung inflammation and mucus production were increased in the airways of IL-12p40-/- mice as compared with those of wild-type mice, concurrent with increased levels of the Th2 effector cytokines IL-5 and IL-13. Respiratory syncytial virus clearance and levels of Th1 effector cytokine IFN-gamma were not altered. Interestingly, IL-18, another mediator of IFN-gamma production, was significantly increased in the lungs of IL-12p40-/- mice early during the course of infection. Abrogation of IL-18-mediated signaling in IL-12p40-/- mice further enhanced Th2 immune response and mucus production in the airways during respiratory syncytial virus infection but failed to modulate IFN-gamma production or viral clearance. These findings implicate a role for IL-12 and IL-18 in modulating respiratory syncytial virus-induced airway inflammation distinct from that of viral clearance.

Topics: Adjuvants, Immunologic; Alcian Blue; Animals; Antibodies, Blocking; Bronchiolitis, Viral; Bronchoalveolar Lavage Fluid; CD3 Complex; Cytokines; Inflammation Mediators; Interferon-gamma; Interleukin-12; Interleukin-12 Subunit p40; Interleukin-18; Leukocyte Count; Lung; Lymphocyte Subsets; Mice; Mice, Inbred BALB C; Mice, Knockout; Mucus; Neutralization Tests; Periodic Acid-Schiff Reaction; Protein Subunits; Respiratory Syncytial Virus Infections; Respiratory Syncytial Viruses; Th2 Cells; Up-Regulation; Viral Load

Clara cell secretory protein (CCSP) has been shown to have anti-inflammatory and immunomodulatory functions in the lung. Respiratory syncytial virus (RSV) is the most common cause of respiratory infection in infants and young children. RSV usually infects small airways and likely interacts with the Clara cells of bronchioles. To determine a possible role for CCSP during acute RSV infection, CCSP-deficient (CCSP(-/-)) and wild-type (WT) mice were intratracheally infected with RSV and the lung inflammatory and immune responses to RSV infection were assessed. RSV-F gene expression was increased in the lungs of CCSP(-/-) mice as compared with WT mice following RSV infection, consistent with increased viral persistence. Lung inflammation was significantly increased in CCSP(-/-) mice as compared with WT mice after infection. Moreover, although the levels of Th1 cytokines were similar, the levels of Th2 cytokines and neutrophil chemokines were increased in the lungs of CCSP(-/-) mice following infection. Physiologic endpoints of exacerbated lung disease, specifically airway reactivity and mucus production, were increased in CCSP(-/-) mice after RSV infection. Importantly, restoration of CCSP in the airways of CCSP(-/-) mice abrogated the increased viral persistence, lung inflammation, and airway reactivity. These findings suggest a role for CCSP and Clara cells in regulating lung inflammatory and immune responses to RSV infection.

Topics: Airway Resistance; Alcian Blue; Animals; Bronchial Hyperreactivity; Chemokines; Cytokines; Gene Deletion; Gene Expression Regulation, Viral; Lung Diseases; Mice; Mice, Knockout; Mucoproteins; Neutrophils; Periodic Acid-Schiff Reaction; Proteins; Respiratory Syncytial Virus Infections; Respiratory Syncytial Viruses; Th2 Cells; Uteroglobin; Virus Replication