alcian-blue and Pulmonary-Edema

alcian-blue has been researched along with Pulmonary-Edema* in 2 studies

Other Studies

2 other study(ies) available for alcian-blue and Pulmonary-Edema

ArticleYear
Anaphylactoid Syndrome of Pregnancy-An Autopsy Case Report.
    The American journal of forensic medicine and pathology, 2021, 06-01, Volume: 42, Issue:2

    Topics: Alcian Blue; Cesarean Section; Coloring Agents; Diabetes, Gestational; Disseminated Intravascular Coagulation; Embolism, Amniotic Fluid; Female; Fetal Membranes, Premature Rupture; Heart Arrest; Humans; Labor, Induced; Lung; Mucins; Pregnancy; Pulmonary Edema; Staining and Labeling

2021
Pleural tissue hyaluronan produced by postmortem ventilation in rabbits.
    Lung, 2000, Volume: 178, Issue:1

    We developed a method that used Alcian blue bound to hyaluronan to measure pleural hyaluronan in rabbits postmortem. Rabbits were killed, then ventilated with 21% O2--5% CO2--74% N2 for 3 h. The pleural liquid was removed by suction and 5 ml Alcian blue stock solution (0.33 mg/ml, 3.3 pH) was injected into each chest cavity. After 10 min, the Alcian blue solution was removed and the unbound Alcian blue solution (supernatant) separated by centrifugation and filtration. The supernatant transmissibility (T) was measured spectrophotometrically at 613 nm. Supernatant Alcian blue concentration (Cab) was obtained from a calibration curve of T versus dilutions of stock solution Cab. Alcian blue bound to pleural tissue hyaluronan was obtained by subtracting supernatant Cab from stock solution Cab. Pleural tissue hyaluronan was obtained from a calibration curve of hyaluronan versus Alcian blue bound to hyaluronan. Compared with control rabbits, pleural tissue hyaluronan (0.21 +/- 0.04 mg/kg) increased twofold, whereas pleural liquid volume decreased by 30% after 3 h of ventilation. Pleural effusions present 3 h postmortem without ventilation did not change pleural tissue hyaluronan from control values. Thus ventilation-induced pleural liquid shear stress, not increased filtration, was the stimulus for the increased hyaluronan produced from pleural mesothelial cells.

    Topics: Alcian Blue; Animals; Epithelium; Hyaluronic Acid; Pleura; Postmortem Changes; Pulmonary Edema; Rabbits; Respiration, Artificial

2000