alcian-blue and Necrosis

We examined the effects of abrupt reperfusion on the mouse submandibular gland parenchyma and determined the degree of recovery from tissue damage. A main trophic artery supplying the gland was ligated with silk thread, and the ligature was then released after a variable period. The gland was removed at various times after reperfusion and then examined immunohistochemically and ultrastructurally. With reperfusion after 15 or 30 min of ligation, the tissue damage to the glands was slight or inapparent. With reperfusion after 1 or 3 h of ligation, collapse of the acini and the ducts was observed in parts of the lobules, but restoration of the parenchymal structures occurred, with the appearance of PCNA-positive cells, although there were differences in the level of restoration. After 6 h of ligation, most of the normal parenchymal cells had disappeared by the 5th and 7th days after reperfusion, and apoptosis and necrosis were present. These findings suggest that if interruption of the blood supply to the submandibular gland parenchyma is limited to within a few hours, then tissue repair after reperfusion is possible, although this will differ according to the level of damage, because the acini and the ducts reappear, probably with proliferation of parenchymal cells.

Topics: Alcian Blue; Animals; Apoptosis; Cell Division; In Situ Nick-End Labeling; Male; Mice; Mice, Inbred ICR; Microscopy, Electron; Necrosis; Proliferating Cell Nuclear Antigen; Reperfusion; Submandibular Gland; Time Factors

We report a case of a 68 years old male patient treated for chronic lymphocytic leukaemia, presenting a right inguino-crural tender lymphadenopathy with periadenitis. The histologic changes associated with adenitis were large foci of necrosis with regular crown of epithelioid cells, without giant cell. The presence of Herpes simplex virus 2 was confirmed by immunohistochemistry and culture. Occurrence, physiopathology, clinical and histological aspects of Herpes simplex lymphadenitis in patients with haematologic disorders, are discussed.

Topics: Aged; Alcian Blue; Culture Techniques; Fluorescent Antibody Technique; Herpes Simplex; Humans; Immunohistochemistry; Leukemia, Lymphocytic, Chronic, B-Cell; Lymphadenitis; Lymphocytes; Male; Necrosis

A new staining technique based on alcian blue and hematoxylin was used during the study of experimentally produced fractures in long bone. The distinction between cartilage, woven bone, mature bone and necrotic bone during successive stages of the fracture healing process was particularly remarkable. This method was found also to be very useful in the study of general tissue morphology. It is suggested that this postdecalcification light microscopy staining method is suitable for studies of cartilage and bone development with particular reference to ossification, remodelling and bone pathology.

Topics: Alcian Blue; Animals; Benzopyrans; Bone and Bones; Bone Development; Bone Resorption; Bony Callus; Cartilage; Cell Differentiation; Fractures, Bone; Hematoxylin; Indoles; Necrosis; Radius Fractures; Rats; Staining and Labeling; Wound Healing