alcian-blue and Nasal-Polyps

Histologic changes have not been systematically assessed in chronic rhinosinusitis. Quantitative histochemical studies evaluated the extent of sinus disease and gland density in the middle turbinates.. Sinus computed tomography scans of 34 patients with chronic rhinosinusitis were retrospectively graded 0 to IV according to the May classification. Middle turbinates from patients with chronic rhinosinusitis (n = 46) and normal patients (n = 7) were harvested during endoscopic sinus surgery. The areas of Alcian blue-stained glands were assessed in paraffin sections using a computer-assisted microscopy video system.. Alcian blue-stained glands occupied 7.94% of normal mucosa. The staining in all grade III rhinosinusitis subjects was increased to 12.94% (P < 0.01). In contrast, grade IV pansinusitis was associated with nasal polyposis (6 of 6) with decreased gland area (3.04%, P < 0.01). When polyp patients were excluded from grade III rhinosinusitis, the Alcian blue-staining area was 17.68% (P < 0.01).. Distinct polypoid and glandular histopathologic patterns are present in chronic rhinosinusitis.

Topics: Alcian Blue; Case-Control Studies; Chronic Disease; Disease Progression; Goblet Cells; Humans; Image Processing, Computer-Assisted; Nasal Mucosa; Nasal Polyps; Rhinitis; Severity of Illness Index; Single-Blind Method; Sinusitis; Tomography, X-Ray Computed; Turbinates

Many batches of alcian blue dye are incompletely soluble at the low pH used for demonstrating mast cells. An improved technique using alcian blue tetrakis (methylpyridium) chloride (alcian blue pyridine variant) is described here. It produces stronger mast cell staining than other alcian blue stains tested.

Topics: Alcian Blue; Humans; Mast Cells; Nasal Polyps; Neurofibroma; Protons; Staining and Labeling; Tolonium Chloride