alcian-blue has been researched along with Mucopolysaccharidosis-I* in 1 studies
1 other study(ies) available for alcian-blue and Mucopolysaccharidosis-I
| Article | Year |
|---|---|
A semi-quantitative micromethod for the determination of free glycosaminoglycans in serum. Results from studies on serum of healthy children of various age and patients affected by mucopolysaccharidosis.
A method is presented for the determination of free glycosaminoglycan (GAG) concentration in as little as 30 microliter serum. By filtration of the serum through DEAE-cellulose paper, the free GAG fraction is selectively adsorbed and concentrated on a circular area of 15.9 mm2; these GAG spots are stained with Alcian Blue. The relationship between the amounts of adsorbed GAG and the optical density of the Alcian Blue spots is linear with a certain range; e.g. for chondroitin sulfate (mixed isomers), from 0.25 to 1.00 micrograms. With this method--which we will refer to as the "DEAE Alcian Blue" method--we estimated the free GAG concentration in sera of individuals of various ages including newborns. Topics: Adolescent; Adult; Aging; Alcian Blue; Child; Child, Preschool; Female; Glycosaminoglycans; Humans; Infant; Infant, Newborn; Infant, Premature; Male; Microchemistry; Middle Aged; Mucopolysaccharidoses; Mucopolysaccharidosis I; Mucopolysaccharidosis III; Spectrophotometry | 1980 |