alcian-blue and Laryngeal-Diseases

A macroscopic method using the dyes Alcian blue and phloxine is described for the investigation of squamous metaplasia in the larynx. The method also demonstrates an intermediate type epithelium about which little is known. The gross staining of the tissues is based upon the differential penetration of the dyes through squamous, intermediate and respiratory epithelia, and on the strong chemical complex formed between Alcian blue and mucin. The method is particularly useful for research purposes owing to the permanency of the staining result.

Topics: Adult; Alcian Blue; Epithelial Cells; Epithelium; Fluoresceins; Humans; Indoles; Laryngeal Diseases; Larynx; Metaplasia; Staining and Labeling; Time Factors

The Alcian blue method is described as an alternative to the use of pyronin for the immediate post-mortem demonstration of squamous in the larynx. Alcian blue-stained specimens can be stored for prolonged periods in 4 per cent formaldehyde in normal saline without loss of contrast and used as wet specimens for teaching purposes. Alcian blue-stained larynges showing squamous metaplasia can be permanently mounted for museum display in either Kaiserling's or Wentworth's fluids.

Topics: Adult; Alcian Blue; Education, Medical; Epithelial Cells; Epithelium; Exhibitions as Topic; Formaldehyde; Frozen Sections; Histological Techniques; Humans; Indoles; Laryngeal Diseases; Larynx; Metaplasia; Museums; Preservation, Biological