alcian-blue and Conjunctival-Diseases

To study histopathological changes in rabbit conjunctiva after suction ring application.. A suction ring was applied to adult albino rabbit eyes (n = 30) with a vacuum pressure of 508 mm Hg for 40 s. Three rabbits were sacrificed at 3 h (3-hour group), 6 h (6-hour group), 1 day (1-day group), 3 days (3-day group), and 7 days (7-day group) after the suction ring application. Histopathological examinations included hematoxylin and eosin, Alcian blue pH 2.5-periodic acid-Schiff (AB2.5-PAS), and Alcian blue pH 1.0-periodic acid-Schiff (AB1.0-PAS) staining. TdT-dUTP-terminal nick-end labeling (TUNEL) assay and immunohistochemical staining for p65 were also performed.. The site of suction ring application and adjacent areas did not show transient inflammation. AB2.5-PAS staining revealed that the percentage of neutral mucin was significantly different between 54.5 +/- 1.6% in the control group and 22.3 +/- 1.3% in the 7-day group (p = 0.04). Apoptosis occurred not only at the site of suction ring application, but also in adjacent regions. At all time points, conjunctival epithelium showed no positive staining for p65.. The transient elevation of pressure induced by a suction ring is sufficient to cause conjunctival damage. These histological results support the finding that conjunctival damage caused by suction ring application is one of many etiologies of dry eye syndrome following laser in situ keratomileusis.

Topics: Alcian Blue; Animals; Apoptosis; Conjunctival Diseases; Goblet Cells; Hydrogen-Ion Concentration; Immunoenzyme Techniques; In Situ Nick-End Labeling; Intraocular Pressure; Keratomileusis, Laser In Situ; Mucins; Periodic Acid-Schiff Reaction; Rabbits; Suction; Synaptotagmin I; Vacuum

Twenty-six subjects with spheroid degeneration were followed up after two years. The number of colourless conjunctival droplets was seen to have increased by on an average 46% (2 alpha = 0.05) and that of autofluorescent conjunctival droplets by 223% (2 alpha less than 0.01). Counting within the individual sites sites disclosed that some droplets will disappear (not less than 30 and 21% respectivelY), while recently formed will constitute at least 76 and 243%. The number of areas with band-shaped keratopathy was found to rise from 3 to 26 (P less than 0.001) out of 104 possibilities, (nasally and temporally of right and left eye). Vital staining (fluorescein, rose bengal, tetrazolium, alcian blue) showed the epithelium above the droplets to be intact, and the droplet-containing eyes were found not to be abnormally dry (break up time, tear production).

Topics: Alcian Blue; Conjunctiva; Conjunctival Diseases; Cornea; Corneal Dystrophies, Hereditary; Fluoresceins; Follow-Up Studies; Humans; Rose Bengal; Staining and Labeling; Tetrazolium Salts; Time Factors