alcian-blue and Chronic-Disease

Histologic changes have not been systematically assessed in chronic rhinosinusitis. Quantitative histochemical studies evaluated the extent of sinus disease and gland density in the middle turbinates.. Sinus computed tomography scans of 34 patients with chronic rhinosinusitis were retrospectively graded 0 to IV according to the May classification. Middle turbinates from patients with chronic rhinosinusitis (n = 46) and normal patients (n = 7) were harvested during endoscopic sinus surgery. The areas of Alcian blue-stained glands were assessed in paraffin sections using a computer-assisted microscopy video system.. Alcian blue-stained glands occupied 7.94% of normal mucosa. The staining in all grade III rhinosinusitis subjects was increased to 12.94% (P < 0.01). In contrast, grade IV pansinusitis was associated with nasal polyposis (6 of 6) with decreased gland area (3.04%, P < 0.01). When polyp patients were excluded from grade III rhinosinusitis, the Alcian blue-staining area was 17.68% (P < 0.01).. Distinct polypoid and glandular histopathologic patterns are present in chronic rhinosinusitis.

Topics: Alcian Blue; Case-Control Studies; Chronic Disease; Disease Progression; Goblet Cells; Humans; Image Processing, Computer-Assisted; Nasal Mucosa; Nasal Polyps; Rhinitis; Severity of Illness Index; Single-Blind Method; Sinusitis; Tomography, X-Ray Computed; Turbinates

To study the prevalence of peptic ulcer and the histological appearance of the gastric mucosa in healthy, asymptomatic Indians infected with Helicobacter pylori.. Asymptomatic, healthy individuals without any GI symptoms were invited to undergo endoscopy of the upper GI tract. A careful search was made for any erosion or ulcer. Four biopsy specimens were obtained from the gastric corpus and antrum. Histological sections were stained with hematoxylin and eosin for histological details and with Loffler's methylene blue for the presence of H. pylori. Alcian blue periodic acid-Schiff stain (pH 2.5) was used to classify and grade areas of intestinal metaplasia.. Histological examination showed chronic gastritis in 72 (80%) persons. Pangastritis was seen in 22% of subjects; pangastritis with antral predominance, in 28%; and antrum-only gastritis, in 50%. Activity was noted in biopsy specimens from only 33% of the subjects. H. pylori infection was present in 70 (78%) subjects. All of these subjects had evidence of chronic gastritis. Endoscopic examination revealed a normal appearance in 88 individuals. In two individuals a duodenal ulcer was seen. Both subjects had severe H. pylori infection in the antral mucosa. Of the 70 persons having H. pylori infection, only two (2.8%) had a duodenal ulcer.. This study showed that despite a high prevalence of H. pylori infection in asymptomatic, healthy Indians, the point prevalence of peptic ulcer is low, and chronic active gastritis is uncommon.

Topics: Adolescent; Adult; Aged; Alcian Blue; Biopsy; Chronic Disease; Coloring Agents; Duodenal Ulcer; Eosine Yellowish-(YS); Female; Fluorescent Dyes; Gastric Mucosa; Gastritis; Gastroscopy; Helicobacter Infections; Helicobacter pylori; Hematoxylin; Humans; India; Intestinal Mucosa; Male; Metaplasia; Methylene Blue; Middle Aged; Periodic Acid-Schiff Reaction; Prevalence; Pyloric Antrum; Stomach Diseases; Stomach Ulcer

The epithelium at the trigone of the urinary bladder showed intestinal metaplasia of a colonic type in three Holstein-Friesian cows affected with chronic polypoid cystitis. Except for Ricinus communis (RCA-I), almost all goblet cells in the whole crypt were positive to periodic acid-Schiff (PAS), alcian blue (AB) pH 2.5, AB pH 1.0, periodate borohydride-potassium hydroxide-PAS (PB-KOH-PAS), Ulex europaeus (UEA-I), Triticum vulgare (WGA) and Arachis hypogaea (PNA) after neuraminidase digestion. This result indicated that most goblet cells contained acidic and neutral glycoconjugates as O-acetylated sialomucin, L-fucose, N-acetyl-glucosamine, neuraminic acid residues and sialic acid-galactose dimers and were devoid of beta-D-galactose. The goblet cells at the surface in the upper half of the crypt contained both sulpho- and sialo-mucins with N-acetyl-galactosamine residues by AB (pH 2.5)-PAS, high iron diamine (HID)-AB (pH 2.5), Dolichos biflorus (DBA) and Glycine maximus (SBA) reactions. On the other hand, the lower goblet cells were found to contain predominantly sulphated mucins with D-mannose and D-glucose residues by AB-PAS, HID-AB and Concanavalia ensiformis (Con A) reactivities. This suggested that mucin secreted from these cells was similar to that secreted from the goblet cells of the large intestine in cattle.

Topics: Alcian Blue; Animals; Carbohydrate Sequence; Cattle; Cattle Diseases; Chronic Disease; Colon; Cystitis; Epithelium; Female; Glycoconjugates; Hydronephrosis; Intestinal Mucosa; Lectins; Metaplasia; Molecular Sequence Data; Mucins; Periodic Acid-Schiff Reaction; Sialoglycoproteins; Urinary Bladder

In children suffering from chronic dermatoses (psoriasis and neurodermatitis), the glycocalix of blood mononuclears displays an Alcian blue dye sorption by 23-25% less than that in healthy children. The UV irradiation of their blood (254 nm), in addition to a course of UV-irradiated blood autotransfusion, resulted in an elevated sorption capacity of the mononuclear glycocalix up to the normal. A possible involvement of these changes in immunocompetent cell glycocalix in the pathogenesis of chronic dermatoses is discussed, as well as the significance of glycocalix normalization in the medicinal effect of UV-irradiated blood autotransfusion.

Topics: Absorption; Adolescent; Alcian Blue; Blood Transfusion, Autologous; Child; Chronic Disease; Combined Modality Therapy; Glycoproteins; Humans; Leukocytes, Mononuclear; Neurodermatitis; Polysaccharides; Psoriasis; Skin Diseases; Surface Properties; Ultraviolet Therapy

Thick mucosa removed from the promontory in cases with chronic otitis media showed prominent PAS-positive glands and epithelial secretory cells. Alcian blue positivity was less pronounced, contrary to the mucosa from glue ears. Enzyme activity in the epithelium and propria was comparable to that in glue ears, with some increase in alkaline phosphatase and some decrease in proprial lactate dehydrogenase and malate dehydrogenase activity. Removal of thick, permanently altered mucoas is recommended even in the absence of squamous epithelium. Steps should be taken to allow regrowth of thin, normal middle ear epithelium on the promontory.

Topics: Acid Phosphatase; Adenosine Triphosphatases; Alcian Blue; Alkaline Phosphatase; Chronic Disease; Ear, Middle; Esterases; Hematoxylin; Humans; L-Lactate Dehydrogenase; Leucyl Aminopeptidase; Malate Dehydrogenase; Mucous Membrane; Nucleotidases; Otitis Media; Staining and Labeling

Vital staining with a tetrazolium - alcian blue mixture gave a characteristic punctate red staining of the superior or the inferior tarsus in 69% of all examined patients with chronic simple conjuctivitis (69 patients subjected to 168 examinations). The superior tarsus was more frequently stained than the inferior (61% against 33%). The staining was most often concentrated centrally or in the middle anterior part of the superior tarsus. If located on the inferior tarsus, it was most often found medially, anteriorly. No corresponding staining was seen in normal eyes. The differential diagnosis involving keratoconjunctivitis sicca and pemphigoid is mentioned. The staining is due to enzymatic reduction of tetrazolium in the cytoplasm of the tarsal epithelial cells. The positive vital staining finding is consistent with a diagnosis of chronic simple conjunctivitis.

Topics: Alcian Blue; Chronic Disease; Conjunctivitis; Humans; Staining and Labeling; Tetrazolium Salts