alcian-blue and Breast-Neoplasms

This report describes an atypical mammary adenoma with a rare histological feature characterized by proliferating single-layered cystic ducts composed of basaloid cells with frequent myoepithelial differentiation. A 9-year-old, intact female Miniature Pinscher dog had mammary tumors on the thorax. Histologically, one of tumors comprised the proliferation of two types of tubular structures; the single-layered cystic ducts lined by flattened cells and double-layered tubules with luminal cells and outer spindle cells. The former ducts were predominant in the tumor and contained pale basophilic mucus, which was Alcian blue (pH 2.5)-positive, but periodic acid Schiff-negative. Immunohistochemical staining indicated that the cells lining single-layered cystic ducts were negative for the luminal epithelial marker, cytokeratin (CK) CAM5.2, but were constantly positive for basal cell markers CK14 and p63 and frequently positive for SMA. Electron microscopy revealed fine, parallel myofilaments within these single-layered neoplastic cells. These histological and immunohistological examinations suggested that the origin of the tumor was bipotential mammary progenitor cells with predominant differentiation into the myoepithelial progenitor linage.

Topics: Adenoma; Alcian Blue; Animals; Biomarkers; Breast Neoplasms; Cell Differentiation; Cell Proliferation; Cystic Duct; Dog Diseases; Dogs; Epithelial Cells; Female; Immunohistochemistry; Keratins; Microscopy, Electron; Myofibrils

The sensitivity of an intraoperative diagnosis of sentinel lymph node metastasis of invasive lobular carcinoma using conventional staining is low.. To develop a fast, modified, Alcian blue stain to decrease the intraoperative false-negative results in testing for metastatic invasive lobular carcinoma.. Modified Alcian blue was optimized. Patients who had invasive lobular carcinoma on needle biopsy were candidates for this study. Touch preparations from every sentinel lymph node were prospectively prepared in the same manner, one stained with modified Alcian blue and one with conventional staining. These slides were independently interpreted.. A total of 121 sentinel lymph nodes from 31 patients with invasive lobular carcinoma were studied. There were 11 patients (35.5%) who had at least one positive lymph node test result. There were a total of 18 positive lymph node results (14.9%). Although 10 sentinel lymph nodes with abnormalities were detected in 7 patients with conventional staining, modified Alcian blue detected 15 sentinel lymph node abnormalities in 10 patients. Modified Alcian blue increased the sensitivity compared with conventional staining from 63.6% to 90.9% (calculated based on the number of patients) and from 55.6% to 83.3% (calculated based on number of sentinel lymph nodes). The staining process takes approximately 11 minutes.. Modified Alcian blue stain is a relatively rapid, cheap, highly sensitive, and specific method of detecting metastatic invasive lobular carcinoma. This method can be used in conjunction with conventional staining methods used intraoperatively.

Topics: Adult; Aged; Aged, 80 and over; Alcian Blue; Breast Neoplasms; Carcinoma, Lobular; Coloring Agents; False Negative Reactions; Female; Humans; Immunohistochemistry; Middle Aged; Monitoring, Intraoperative; Neoplasm Metastasis; Prospective Studies; Sentinel Lymph Node Biopsy

Topics: Adenocarcinoma; Aged; Alcian Blue; Amylases; Biomarkers, Tumor; Breast Neoplasms; Chromosomes, Human, Pair 12; Chromosomes, Human, Pair 15; Coloring Agents; Diagnosis, Differential; Female; Glycogen; Hormone Replacement Therapy; Humans; Middle Aged; Neoplasm Proteins; Oncogene Proteins, Fusion; Periodic Acid-Schiff Reaction; Prognosis; Translocation, Genetic

To compare the shape of the sonographic image of invasive breast cancer with the shape of the corresponding histopathologic section stained for components of the extracellular matrix, hyaluronan, and collagen.. We studied 22 cases of breast carcinoma. Among them, 13 were visible on both sonography and mammography, whereas 9 were visible only on sonography. We compared the sonographic images with the histologic sections of the same carcinomas after staining with alcian blue for hyaluronan and Masson trichrome for collagen.. We have shown that the shape of the sonographic image of breast cancer is similar to the shape of its hyaluronan extracellular matrix.. This finding explains why breast sonography is so sensitive for detection of invasive carcinoma and why some invasive cancers are not visible on mammography.

Topics: Alcian Blue; Breast Neoplasms; Carcinoma, Ductal, Breast; Coloring Agents; Extracellular Matrix; Female; Histocytochemistry; Humans; Hyaluronic Acid; Middle Aged; Ultrasonography, Mammary

Localization of metastases within the sentinel lymph nodes (SLNs) of breast cancer has not been studied. Forty SLNs from 36 patients with operable primary breast cancers were identified by means of lymphatic mapping with patent blue dye. The junction between the patent blue-stained lymphatic vessel draining the tumor and the SLN was labeled with alcian blue. Metastases within the serially sectioned SLNs were assigned to the alcian blue-labeled side, to the opposite side of the virtually halved nodes, or both. Eight SLNs were negative for metastasis. Eleven SLNs had metastases only in the blue half. Only 4 cases had larger metastases in the nonblue half. Metastases are more likely to be located in the vicinity of the inflow junction of the identifiable lymphatic draining the tumor and the SLN. This should be considered when SLNs are examined, especially when they are halved for different studies.

Topics: Alcian Blue; Axilla; Breast Neoplasms; Female; Humans; Immunohistochemistry; Lymph Node Excision; Lymph Nodes; Lymphatic Metastasis; Specimen Handling; Staining and Labeling

Three cases of nodular mucinosis of the breast are presented. They occurred in one male and two female patients. The patients had no signs of Carney's syndrome. All lesions were located under the nipple. They were poorly circumscribed and unencapsulated. They were soft and had a gelatinous consistency and white color. No patient had any evidence of recurrence or metastasis 6 months, 3 years and 6 years, respectively, after the surgical excision. The mucinous tissue consisted of acid mucopolysaccharides, which stained Alcian blue, Hale's colloidal iron positively, and they were periodic acid-Schiff (PAS) negative. Mucicarmine reaction was only faintly positive. The lesion should be distinguished particularly from mucocele-like lesions of the breast by the location, nodular arrangement, absence of accompanying ductal hyperplasia and staining properties of the mucous substances.

Topics: Adenocarcinoma, Mucinous; Adult; Alcian Blue; Breast Neoplasms; Breast Neoplasms, Male; Carcinoma, Ductal, Breast; Diagnosis, Differential; Female; Histocytochemistry; Humans; Ki-67 Antigen; Male; Mucocele; Periodic Acid-Schiff Reaction

Topics: Adenocarcinoma; Alcian Blue; Breast Neoplasms; Carcinoma, Small Cell; Carcinoma, Squamous Cell; Cytodiagnosis; Female; Humans; Lung Neoplasms; Ovarian Neoplasms; Periodic Acid-Schiff Reaction; Staining and Labeling; Stomach Neoplasms

The authors evaluate the distribution and the content of glycosaminoglycans (GAG) in neoplastic tissue from mastectomy specimens of 15 patients treated for an infiltrating ductal carcinoma NOS, in comparison to normal tissue of the opposite quadrant. Neoplastic tissues contain a marked amount (5 mg/g dry defatted tissue) of GAG, in comparison to normal ones (2.2 mg/g dry defatted weight). In stellate carcinomas GAG are localized near the interface with the host tissue and are constituted mainly of chondroitin sulfates and hyaluronate. These data are confirmed by electrophoretic analysis.

Topics: Alcian Blue; Breast; Breast Neoplasms; Carcinoma, Intraductal, Noninfiltrating; Electrophoresis, Cellulose Acetate; Female; Glycosaminoglycans; Humans; Middle Aged

Topics: Adult; Aged; Alcian Blue; Amyloid; Breast Neoplasms; Female; Glycosaminoglycans; Histocytochemistry; Humans; Middle Aged; Periodic Acid; Staining and Labeling