alcian-blue and Bone-Neoplasms

Viscous material secreted from NMSG 10 cells cultured from malignant fibrous histiocytoma of human bone was demonstrated using two-dimensional electrophoresis to be glycosaminoglycans (GAGs) consisting of mainly hyaluronic acid (HA), along with small amounts of heparan sulfate (HS) and chondroitin sulfate (ChS). The biosynthesis of HA in a cell-free system was located at the plasma membrane fraction by colloidal-iron-reactive ultrastructural staining. The molecular weight of the HA synthesized by the plasma membrane fraction was estimated to be about 90,000 D by gel filtration. The material secreted by the original tumor tissue of NMSG 10 cells was demonstrated to be GAGs consisting of HA, HS, ChS and dermatan sulfate using two-dimensional electrophoresis. These findings suggest that transformed tumor cells could synthesize HA at the plasma membrane and release it into the stroma of tumor tissue.

Topics: Alcian Blue; Bone Neoplasms; Cell Membrane; Cell-Free System; Chondroitin Sulfates; Dermatan Sulfate; Heparitin Sulfate; Histiocytoma, Benign Fibrous; Humans; Hyaluronic Acid; Hyaluronoglucosaminidase; Staining and Labeling; Tumor Cells, Cultured

Electron-microscopic studies in which Alcian blue was used to demonstrate proteoglycans (PG) revealed a predominance of largely undifferentiated tumor cells in two of the osteosarcomas examined; the extracellular matrix of these tumors had few PG-containing structures. Other osteosarcomas, where cells of the osteoblastic type prevailed, were found to possess PG granules on the cell surface and a rough PG network in the pericellular space. In a parosteal osteosarcoma, which contained strongly elongated fibroblast-like cells, PG formed a fine mesh both in the pericellular space and throughout the extracellular matrix. A correlation therefore exists between the ultrastructure of the PG component in the extracellular matrix of osteogenic sarcomas and the degree and direction of tumor cell differentiation.

Topics: Alcian Blue; Animals; Bone Neoplasms; Dog Diseases; Dogs; Extracellular Matrix; Microscopy, Electron; Osteosarcoma; Proteoglycans

The present investigation endeavors to characterize the mucosubstance content of 170 myxoid and chondromatous tumors and chordomas by histochemical methods. The results obtained using the critical electrolyte concentration (CEC) method as introduced by Scott and co-workers23,24 were compared with those obtained by staining with alcian blue and toluidine blue at different pH's with and without pretreatment with bovine testicular hyaluronidase. Tissues known biochemically to contain different heteroglycans were used as controls: synovial fluid and cock's comb (hyaluronic acid) stained with alcian blue up to a MgCl2 concentration of 0.1 M; fetal cartilage (chondroitin 4- and 6-sulphate) pulposus with notochordal remnants (keratan sulphate) up 10 1.0 M. The staining reaction of intramuscular myxoma and myxoid liposarcoma corresponded to that of synovial fluid and cock's comb (containing hyaluronic acid). Benign chondromatous tumors (osteochondroma, enchondroma, extraskeletal chondroma, chondromatosis in bursae, synovia, and tendon) as well as well-differentiated chondrosarcomas had a similar staining reaction to that of adult cartilage (containing keratan sulphate). However, the intensity of the reaction was lower in these tumors than in the adult cartilage, indicating that the keratan sulphate content of the tumors is lower. Most of the moderately well-differentiated chondrosarcomas, the poorly differentiated chondrosarcomas, and pulmonary metastases of chondrosarcoma, as well as mesenchymal chondrosarcoma and extra-skeletal chondrosarcoma possessed the same staining properties as fetal cartilage, known to contain chondroitin 4- and 6-sulphate but not keratan sulphate. A few of the moderately well-differentiated chondrosarcomas stained up to a MgCl2 concentration of 1.0 M. Three cases of poorly differentiated chondrosarcomas stained with alcian blue up to 0.35-0.45 M in the lowest differentiated areas, indicating the presence of sulphated heteroglycans, as chondroitin 4- and 6-sulphate. Most chordomas possessed the same staining properties as fetal cartilage; however, a few chordomas stained in the same way as notochordal remnants of nucleus pulposus (containing keratan sulphate), which are thought to be the origin of these tumors. The results of staining of the tumors in the present series with the Scott technique corresponds well with toluidine blue and alcian blue at different pH's with and without pretreatment of the sections with testicular hyaluronidas

Topics: Adult; Aged; Alcian Blue; Bone Neoplasms; Child; Chondroitin Sulfates; Chondroma; Chondrosarcoma; Chordoma; Glycosaminoglycans; Histocytochemistry; Humans; Hyaluronic Acid; Hyaluronoglucosaminidase; Hydrogen-Ion Concentration; Keratan Sulfate; Liposarcoma; Myxoma; Osmolar Concentration; Soft Tissue Neoplasms; Tolonium Chloride