agar and Vaginitis

A selective and differential agar for optimal growth of the large curved motile anaerobic rods isolated from patients having non-specific vaginosis (NSV) was developed. A basal medium of Columbia CNA agar was used with colony growth found to be optimal by the addition of 7% fetal calf serum and 5% rabbit blood (CNARS). Beta-Haemolysis was found to be demonstrated most readily on a bilayer plate with a basal layer consisting of CNA agar with fetal calf serum, overlaid with a layer of CNA agar containing fetal calf serum and rabbit blood. Optimal growth was found by anaerobic incubation at 37 degrees C, after 5 days.

Topics: Agar; Bacteria, Anaerobic; Bacteriological Techniques; Culture Media; Female; Humans; Vaginitis

Topics: Actinomycetales; Agar; Animals; Blood; Culture Media; Female; Gardnerella vaginalis; Haemophilus; Haemophilus Infections; Humans; In Vitro Techniques; Sheep; Vagina; Vaginitis

The principal objective of this study was the characterization of Gardnerella vaginalis by gas chromatography. Thirty-eight isolates and the type strain, ATCC 14018, of G. vaginalis were studied. Hexadecanoic (16:0), octadecenoic (18:1) and octadecanoic (18:0) were the major fatty acids detected. Only insignificant differences between the various isolates could be found. The gas chromatographic analysis of G. vaginalis revealed a characteristic pattern. Gas chromatography in combination with selective growth conditions provides a method for rapid and exact identification.

Topics: Agar; Animals; Blood; Chromatography, Gas; Fatty Acids; Female; Gardnerella vaginalis; Haemophilus; Haemophilus Infections; Hemolysis; Humans; In Vitro Techniques; Sheep; Vaginitis

Topics: Adolescent; Agar; Cervix Uteri; Coitus; Culture Media; Female; Gonorrhea; Humans; Neisseria gonorrhoeae; Vaginal Smears; Vaginitis