agar and Uterine-Neoplasms

Four families of human tumour cell lines--one of uterine, and three of ovarian origin--were established at early passage level from primary biopsy specimens of terminal patients by the propagation of anchorage-independent agar clones in liquid culture. All cell lines exhibited unique and stable characteristics and retained their ability to clone in agar. However, considerable heterogeneity was evident in clonogenic capacity, karyotype, and responsiveness to growth-promoting substances even among progeny of single agar clones isolated from the one biopsy specimen. These cell lines will be made available for further study upon request.

Topics: Agar; Aged; Biopsy; Carcinoma; Cell Line; Culture Media; Cystadenocarcinoma; Cytological Techniques; Female; Humans; Middle Aged; Neoplasms; Neoplasms, Experimental; Ovarian Neoplasms; Uterine Neoplasms

Cellular growth interactions were studied between neonatal human lung fibroblasts (NLF-13) and human tumor lines derived from carcinomas of the prostate (PC-3, DU145), bladder (J82), and endometrium (HEC-1A) and from a glioma (Hs 683t). NLF-13 were interacted with tumor cells in soft agar or agarose media using two experimental protocols. In one system, NLF-13 cells were grown as anchored monolayers proliferating under the tumor cell layer. In the second, NLF-13 were embedded directly (nonanchored) into the agar or agarose layer with the tumor cells. The results from both interaction systems were similar for all five tumor lines. Anchored NLF-13 caused a dose-dependent inhibition of tumor growth, whereas nonanchored cells produced a dose-dependent growth stimulation. A time exposure experiment indicated that tumor stimulation and inhibition were biphasic responses to NLF-13. It was concluded that low concentrations of a diffusible NLF-13 product(s) accelerated tumor growth, whereas high concentrations were inhibitory. Further, the production of the active NLF-13 substance(s) was positively correlated with NLF-13 growth rate. Tumor cell inhibition was irreversible after a 5-day exposure to proliferating NLF-13 cells. Another line of normal neonatal human lung fibroblasts (NLF-147) showed inhibitory properties similar to those described for NLF-13. However, preliminary studies with fibroblasts from the skin of a Down's syndrome neonate (DS-172) and from a human kidney tumor (KTF-130) have shown both these fibroblast types to have a reduced ability to inhibit tumor cell cultures (J82) compared to the neonatal lung fibroblasts (NLF-13 and NLF-147).

Topics: Agar; Cell Communication; Cell Division; Cell Line; Female; Fibroblasts; Glioma; Humans; Infant, Newborn; Kinetics; Lung; Male; Prostatic Neoplasms; Urinary Bladder Neoplasms; Uterine Neoplasms

Topics: Adenocarcinoma; Adolescent; Adult; Agar; Aged; Blood Protein Electrophoresis; Endometriosis; Female; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leiomyoma; Leiomyosarcoma; Middle Aged; Polyps; Spectrophotometry; Uterine Neoplasms