agar and Ulcer

To report a new entity called "toxic non-inflammatory fungal keratitis.". Eyes manifesting infective keratitis with a history of prior administration of topical steroids were included in the study. The details pertaining to the type of injury, duration of injury, and primary treatment for corneal trauma were meticulously documented. The corneal tissues were scraped from the patients and were analyzed for fungal filaments by using a 10% KOH mount under a compound microscope. Moreover, these scraped materials were plated on blood agar and Sabouraud dextrose agar plates.. The corneal ulcers displayed a disproportionately reduced intensity of pain and improved visual acuity. Further, 10% KOH revealed profuse fungal filaments with few inflammatory cells in all the patients. The anterior chamber cells and flare were either reduced or entirely absent. There was no evidence of lid edema and surrounding corneal edema in any of the patients. The mean healing period was 28.8 days (standard deviation (SD): 10.05). The KOH mount revealed the presence of confluent fungal hyphae with a few inflammatory cell infiltrates. The Aspergillus species and Fusarium species were found in 47% and 40% of the cases, respectively.. Toxic non-inflammatory fungal keratitis following steroid therapy needs to be considered in fungal ulcers with disproportionately less pain and good visual acuity. The fungal ulcers with altered clinical signs of classical inflammation need to be assessed for topical steroid misuse.

Topics: Agar; Cornea; Corneal Ulcer; Eye Infections, Fungal; Fungi; Humans; Keratitis; Pain; Steroids; Ulcer

Fusarium species are saprophytic fungi with a worldwide distribution. These fungi cause various infections among immunocompromised patients; however, they can also involve immunocompetent individuals.. We report a case of a 41-year-old Iranian woman who presented with ulcerative lesions on her lips 10 months ago. She had a long history of anxiety but had no history of classical risk factors such as trauma, cosmetic lip tattoo, burning in her lips, smoking or use of alcohol and opium. A skin biopsy from the lower lip was performed and sent for microbiological examinations. Hyaline septate hyphae were seen on direct microscopy with potassium hydroxide. The clinical specimen was subcultured on sabouraud dextrose agar with chloramphenicol and prepared for antifungal susceptibility testing and molecular identification. Considering the minimum inhibitory concentrations (MIC) for antifungals, itraconazole (100 mg orally twice a day) was started for her, and after 2 months, the lesions were treated. She followed up for 3 months, and no signs of disease recurrence were observed.. Selecting an appropriate treatment strategy according to the laboratory assessments is essential in clinical practice and the management of rare infections to prevent related mortality and morbidity of opportunistic fungal infections.

Topics: Adult; Agar; Antifungal Agents; Chloramphenicol; Female; Fusarium; Glucose; Humans; Iran; Itraconazole; Lip; Opium; Ulcer

Topics: Agar; Animals; Carrageenan; Colonic Diseases; Guinea Pigs; Male; Rhodophyta; Ulcer

Topics: Agar; Bacteriocins; Bacteriological Techniques; Bacteriuria; Blood; Bone and Bones; Cross Infection; Humans; Mitomycins; Pseudomonas aeruginosa; Pseudomonas Infections; Sputum; Tracheotomy; Ulcer; Wound Infection

Commercially available sealed blood-agar plates have been demonstrated to retain their usefulness for as long as 3 months under jungle conditions without refrigeration.

Topics: Agar; Bacteria; Bacteriological Techniques; Blood; Culture Media; Humans; Tropical Climate; Ulcer