agar and Tonsillitis

The interference between alpha-streptococcal strains obtained from patients with repeated tonsillitis and a collection of group A streptococcal strains were studied. For this purpose three in vitro methods were designed and compared. The results obtained by a simple plating technique suitable for screening purposes were found to correlate well with those using more laborious techniques. In a limited scale some of the alpha- and beta-streptococcal combinations were tested under in vivo conditions using a tissue cage model allowing repeated sampling. In most instances agreement between the results of the in vitro and in vivo methods was registered. Several alpha-strains having inhibitory capacity to the majority of a collection of group A streptococci belonging to different serotypes were found, but also alpha-strains with an inhibitory activity restricted to few group A isolates within a certain serotype. Also beta-streptococcal strains with growth inhibiting activity towards some alpha-strains were found. As the methods were chosen to eliminate many of the unspecific inhibitory factors and the beta-hemolytic test strains showed a pattern of inhibition that varied for each of the reference alpha-strains the activity is most likely attributed to bacteriocin-like substances.

Topics: Adolescent; Adult; Agar; Animals; Antibiosis; Child; Humans; Middle Aged; Palatine Tonsil; Rabbits; Species Specificity; Streptococcus; Streptococcus pyogenes; Streptococcus sanguis; Tonsillitis

1.2 mega U Penicillin V potassium was given to 20 patients 2 hours (group 1) and another 19 patients 3 hours (group 2) before tonsillectomy. After the operation biological determinations of the levels of active principle were made from tonsillar tissue and serum with statistical evaluation of the results (Spearman's rank correlation). There was a significant connection between the serum and tonsillar tissue concentrations (in children and adults) in both groups. The level of the active principle in group 2 was only slightly lower than in group 1. Many of the micro-organisms responsible for tonsillitis can be influenced by the concentrations found here, so that application of Penicillin V can often be expected to have a good therapeutic effect.

Topics: Adolescent; Adult; Agar; Bacillus subtilis; Child; Child, Preschool; Female; Humans; Male; Middle Aged; Palatine Tonsil; Penicillin V; Potassium; Streptococcus pyogenes; Time Factors; Tonsillectomy; Tonsillitis

Topics: Agar; Blood Protein Electrophoresis; Chronic Disease; Gels; Humans; Tonsillitis

Topics: Adolescent; Adult; Agar; Cephalosporins; Child; Drug Resistance, Microbial; Electrolytes; Female; Humans; Liver; Male; Middle Aged; Otitis Externa; Otitis Media; Staphylococcal Infections; Staphylococcus; Streptococcal Infections; Tonsillitis

Topics: Adolescent; Adult; Agar; Cholecystitis; Chronic Disease; Diagnosis, Differential; Female; Glycoproteins; Heart Diseases; Humans; Immunodiffusion; Male; Methods; Rheumatic Diseases; Tonsillitis

Topics: Agar; Antistreptolysin; Aortic Valve Stenosis; Arthritis; Dextran Sulfate; Dextrans; Glomerulonephritis; Humans; Immune Sera; Mitral Valve Stenosis; Osteomyelitis; Rheumatic Fever; Staphylococcal Infections; Streptococcal Infections; Tonsillitis

Topics: Agar; Animals; Bacteriology; Blood; Humans; Pharyngitis; Sheep; Streptococcus; Tonsillitis