agar and Sarcoma

By a new procedure stable monolayer cultures were derived from spheroids in 8 out of 17 different human sarcomas (16 soft-tissue and I osteogenic sarcoma). Eleven of the sarcomas were obtained from patients undergoing surgery, and 6 from BALB/c nude mice carrying s.c. growing xenografts. The new procedure involves aggregation of single-cell suspensions into spheroids and cultivation of these in agar-coated flasks until the growth rate levels off, at which time the spheroids are transferred to uncoated flasks. Cells proliferating from the rim of adhering spheroids are trypsinized and aggregated to form new spheroids. By 3 to 5 such alternations, monolayer cultures were obtained that have now been subcultured for about 6 months. The cell lines all gave rise to colonies in a clonogenic soft-agar system, and upon s.c. injection into athymic nude mice 3 lines tested formed growing tumors. The histology of spheroids formed from late monolayer passages closely resembled that of the original tumors. That the new procedure is superior to other methods of establishing sarcoma cell lines is indicated by the fact that a stable monolayer culture could be obtained directly from the tumors in only 1/8 cases where the above procedure was successful, and in only 2 instances from soft-agar colonies derived from the tumors.

Topics: Adolescent; Adult; Agar; Aged; Animals; Cell Line; Cells, Cultured; Female; Humans; Male; Mice; Mice, Nude; Microscopy, Electron; Middle Aged; Sarcoma; Sarcoma, Experimental; Staining and Labeling

The correlation of the colony growth of cells disaggregated from human melanoma, sarcoma, lung, and ovarian carcinomas were studied in four different semisolid tissue culture assays: (a) the soft agar assay of Pluznik and Sachs; (b) the soft agar assay of Hamburger and Salmon; (c) the soft agar-methyl cellulose assay of Buick et al.; and (d) the methyl cellulose assay of Ogawa et al. There was no colony growth of tumor cells achieved in 15 of 15 cases assayed in Ogawa's methyl cellulose assay. The plating efficiency of the above mentioned tumors was similar in the assays of Pluznik and Sachs, Hamburger and Salmon, and Buick et al. However, the tumor take rate differed among these three systems. The assay of Buick et al. appears potentially useful for analysis of the biology of human tumors.

Topics: Adenocarcinoma; Agar; Cell Division; Cells, Cultured; Cytological Techniques; Female; Humans; Lung Neoplasms; Melanoma; Methylcellulose; Ovarian Neoplasms; Sarcoma

The effect of mechanical and enzymatic disaggregation on human malignant melanoma, soft-tissue sarcoma and lung carcinoma colony growth in soft agar was studied. The enzymatic disaggregation was advantageous in most cases of melanoma and sarcoma, giving a larger number of colonies and increasing the probability of achieving growth in soft agar. Enzymatically treated pulmonary carcinoma cell populations had lower clonogeneic potential, especially in the case of anaplastic carcinomas. Morphological studies showed that the cells growing in soft-agar colonies had the same characteristics as those of the original tumor. A linear relationship was obtained between the number of enzymatically and mechanically treated tumor cells plated and the number of colonies. Delayed plating decreased the number of colonies.

Topics: Agar; Carcinoma; Cell Aggregation; Clone Cells; Cytological Techniques; Humans; Lung Neoplasms; Melanoma; Microbial Collagenase; Sarcoma; Soft Tissue Neoplasms; Time Factors

Topics: Agar; Carcinoma; Electrophoresis; Gels; Half-Life; Hodgkin Disease; Humans; Isoenzymes; L-Lactate Dehydrogenase; Leukemia, Myeloid; Leukemia, Myeloid, Acute; Leukocytes; Lymphatic Diseases; Lymphoma; Neoplasms; Osteosclerosis; Plasmacytoma; Sarcoma; Tissue Extracts

Topics: Agar; Animals; Cricetinae; Culture Techniques; Histocompatibility Testing; Methods; Microscopy, Phase-Contrast; Sarcoma; Sarcoma, Experimental; Suspensions

Topics: Agar; Alkaline Phosphatase; Bile; Bone Diseases; Bone Neoplasms; Child; Clinical Enzyme Tests; Electrophoresis; Hodgkin Disease; Humans; Jaundice; Jaundice, Obstructive; Liver Diseases; Liver Neoplasms; Lymphoma, Large B-Cell, Diffuse; Lymphoma, Non-Hodgkin; Osteitis Deformans; Sarcoma

Topics: Agar; Animals; Neoplasms; Neoplasms, Experimental; Sarcoma; Sarcoma, Avian; Sarcoma, Experimental

Topics: Agar; Animals; Chickens; Humans; Neoplasms; Sarcoma

Topics: Agar; Animals; Antigens; Chickens; Neoplasms; Sarcoma; Sarcoma, Avian

Topics: Agar; Animals; Antigens; Chickens; Humans; Neoplasms; Sarcoma; Software