agar has been researched along with Salmonella-Food-Poisoning* in 6 studies
6 other study(ies) available for agar and Salmonella-Food-Poisoning
Article | Year |
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An accelerated method for isolation of Salmonella enterica serotype Typhimurium from artificially contaminated foods, using a short preenrichment, immunomagnetic separation, and xylose-lysine-desoxycholate agar (6IX method).
Rapid isolation of Salmonella from food is essential for faster typing and source tracking in an outbreak. The objective of this study was to investigate a rapid isolation method that would augment the standard U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) method. Food samples with low microbial load, including egg salad and ice cream, moderately high-microbial-load tomatoes, and high-microbial-load ground beef were intentionally inoculated with 2 to 48 CFU of Salmonella enterica serotype Typhimurium. The samples were preenriched in buffered peptone water for 6 h, and then selectively concentrated by immunomagnetic separation and plated for isolation on xylose-lysine-desoxycholate agar: the 6IX method. Salmonella Typhimurium was presumptively identified from approximately 97% of the low-microbial-load and moderately high-microbial-load samples by the 6IX method 2 days before the BAM standard method for isolation of Salmonella. In 49% of the beef samples, Salmonella Typhimurium was presumptively identified 1 or 2 days earlier by the 6IX method. Given the inocula used, our data clearly indicated that for most of the food samples tested, with the exception of ground beef, Salmonella Typhimurium could be isolated two laboratory days earlier with the 6IX method compared with the BAM method. In conclusion, this 6IX method may expedite Salmonella isolation and, therefore, has the potential to accelerate strain tracking for epidemiological analysis in a foodborne outbreak. Topics: Agar; Colony Count, Microbial; Consumer Product Safety; Deoxycholic Acid; Disease Outbreaks; Food Contamination; Food Microbiology; Humans; Immunomagnetic Separation; Lysine; Salmonella Food Poisoning; Salmonella typhimurium; Sensitivity and Specificity; Species Specificity; Time Factors; Xylose | 2009 |
Effect of selective growth media on the differentiation of Salmonella enterica serovars by Fourier-Transform Mid-Infrared Spectroscopy.
Salmonella enterica serovars are prevalent foodborne pathogens responsible for high numbers of salmonellosis each year. Complex Fourier-transform infrared (FTIR) spectra offer unique biochemical fingerprints of bacteria with bands due to major cellular components. Growth media effects on discrimination of Salmonella serovars by FTIR spectroscopy were investigated and a novel sample preparation technique was developed. S. enterica strains from six serovars were grown on xylose lysine desoxycholate (XLD), Miller-Mallinson (MM), and plate count (PCA) agar as a control (37 degrees C, 24 h). Isolated colonies were suspended in 50% acetonitrile and centrifuged; the remaining pellet was placed on an AMTIR (attenuated total reflectance) crystal and dried under vacuum. Classification models (Soft Independent Modeling of Class Analogy, SIMCA), generated from derivatized infrared spectra (1300-900 cm-1 or 1200-900 cm-1), successfully discriminated among Salmonella strains with major discrimination from 1000-970 cm-1 associated to stretching modes of O-specific polysaccharide chains of lipopolysaccharides. Sample treatment with acetonitrile enhanced safe handling of the bacteria, removed interfering signals and improved the discriminating ability of SIMCA. All media were able to discriminate the S. enterica strains studied, varying in discriminating peaks and class distances in SIMCA classification. This methodology, with the production of large libraries of pathogenic bacteria, could be applied for the rapid monitoring of bacterial contamination in food with minimal sample manipulation. Topics: Agar; Bacteriological Techniques; Culture Media; Food Microbiology; Humans; Lipopolysaccharides; Multivariate Analysis; Salmonella enterica; Salmonella Food Poisoning; Spectroscopy, Fourier Transform Infrared | 2007 |
[Recent developments in the diagnosis of bacterial food intoxications (author's transl)].
Topics: Agar; Botulism; Clostridium botulinum; Clostridium perfringens; Enterotoxins; Foodborne Diseases; Humans; Salmonella Food Poisoning; Staphylococcal Food Poisoning; Staphylococcus; United Kingdom; United States | 1974 |
Evaluation of malonate dulcitol lysine iron agar for presumptive identification of Salmonellae.
Topics: Agar; Carboxy-Lyases; Cells, Cultured; Culture Media; Evaluation Studies as Topic; Galactitol; Humans; Iron; Lysine; Malonates; Methods; Salmonella; Salmonella Food Poisoning; Serotyping; Sugar Alcohols | 1974 |
An evaluation of strontium chloride, Rappaport and strontium selenite enrichment for the isolation of salmonellas from man, animals, meat products and abattoir effluents.
Strontium chloride enrichment broth was found to be comparable to Rappaport broth for the recovery of a wide range of Salmonella serotypes from man, animals, meat products and effluents. With the exception of cloacal samples from reptiles, both procedures were superior to selenite F.The performance of strontium chloride Mand selenite F enrichment was improved when effluent samples were incubated at 43 degrees C.Strontium chloride M and Rappaport enrichment were superior to selenite F for the isolation of Arizona species from reptiles.Strontium chloride B, strontium selenite and Rappaport broths were found suitable for the isolation of multiple Salmonella serotypes from sea water contaminated with abattoir effluents. The strontium chloride B and strontium selenite enrichment media were superior to Rappaport broth when samples were incubated at 43 degrees C.Modified bismuth sulphite agar was found superior to Salmonella-Shigella agar as a solid subculture medium.The investigation of a food poisoning outbreak due to Salmonella typhimurium phage type 21 is reported.The significance of the choice of sampling and isolation techniques in salmonellosis in man and animals is discussed. Topics: Abattoirs; Agar; Animals; Bismuth; Cats; Cattle; Cloaca; Culture Media; Disease Outbreaks; Dogs; Food Microbiology; Guinea Pigs; Hot Temperature; Humans; Meat; Mice; Rats; Reptiles; Salmonella; Salmonella Food Poisoning; Seawater; Strontium; Water Microbiology | 1972 |
SALMONELLOSIS IN MICE-DIAGNOSTIC PROCEDURES.
Topics: Agar; Animals; Bacteriological Techniques; Clinical Laboratory Techniques; Cystine; Feces; Mice; Research; Salmonella Food Poisoning; Salmonella Infections; Salmonella Infections, Animal; Selenium; Veterinary Medicine | 1963 |