agar and Retinoblastoma

Multifunctional nanoparticles are next generation materials that can be simultaneously used for imaging, diagnosis, and delivery of drugs. However, materials intended for cancer diagnosis need to be investigated for its cell uptake, toxicity, and effectiveness. In the current work, we have synthesized fluorescent iron oxide nanoparticles and evaluated its efficacy against retinoblastoma cell imaging. The iron oxide nanoparticles were synthesized and stabilized using oleic acid. Sulforhodamine B was adsorbed onto albumin over the oleic acid-capped iron oxide nanoparticles. Our results demonstrated good cell uptake in a time-dependent manner and nanoparticles were found to localize in the cytosol. Further, the nanoparticles exhibited excellent negative contrast in magnetic resonance imaging (MRI) experiments and with no cytoxicity (5-100 μg/mL iron oxide nanoparticles) to both normal as well as cancer cells demonstrating its biocompatibility. Thus, this novel material integrates the ability to image tissues with high sensitivity by MRI and specifically visualize Y79 retinoblastoma cells by fluorescence imaging with no toxicity.

Topics: Agar; Albumins; Amines; Cell Line, Tumor; Cell Survival; Contrast Media; Cytosol; Ferric Compounds; Fluorescent Dyes; Humans; Magnetic Phenomena; Magnetic Resonance Imaging; Materials Testing; Microscopy, Fluorescence; Multimodal Imaging; Nanoparticles; Oleic Acid; Phantoms, Imaging; Retinoblastoma; Rhodamines

The effect of various components of three semi-solid media on colony formation in two representative retinoblastoma cell lines, Y-79 and WERI, was determined. Diethylaminoethyl dextran was found to be toxic to the cells, and was deleted from the medium. Horse serum was also used without heat treatment. In the most improved culture medium, plating efficiency was 28% for Y-79 cells and 14% for WERI cells, an increase of more than 10 times that of the original formula. In the new medium, both Y-79 and WERI cells showed relatively constant plating efficiency within a certain range, showing that the medium is useful for quantitative clonogenic study of retinoblastoma cells.

Topics: Agar; Animals; Blood Physiological Phenomena; Cell Line; Culture Media; DEAE-Dextran; Eye Neoplasms; Horses; Hot Temperature; Humans; Neoplastic Stem Cells; Retinoblastoma

Topics: Agar; Amniotic Fluid; Animals; Cattle; Cell Line; Child; Chondrosarcoma; Choroid Neoplasms; Conjunctiva; Culture Media; Culture Techniques; Densitometry; Edetic Acid; Electrophoresis; Female; HeLa Cells; Humans; Immune Sera; Isoenzymes; L-Lactate Dehydrogenase; Liver; Neoplasms, Germ Cell and Embryonal; Organ Culture Techniques; Ovary; Penicillins; Pterygium; Retinoblastoma; Skin; Streptomycin; Umbilicus