agar and Pneumonia

Legionnaires' disease is an atypical pneumonia caused by inhaling small droplets of water containing the bacterium Legionella spp. In newborns, it is a rare event, usually associated with water births and the use of air conditioning systems or air humidifiers. A nosocomial outbreak of Legionnaires' disease in the maternity ward of a secondary-care hospital in Arandjelovac, Serbia is described.Two male newborns were found to be infected with

Topics: Agar; Charcoal; Cycloheximide; Disease Outbreaks; Female; Glycine; Humans; Infant, Newborn; Legionella pneumophila; Legionnaires' Disease; Male; Pneumonia; Polymyxins; Pregnancy; Serbia; Vancomycin; Water; Water Microbiology

Alginate-producing, mucoid P. aeruginosa is frequently found in the lungs of patients with cystic fibrosis (CF), where it causes a chronic infection. The importance of alginate in the pathogenesis was demonstrated by the ability to establish chronic P. aeruginosa lung infection in rats if P. aeruginosa entrapped in minute alginate-beads were inoculated transtracheally. Alginate beads containing P. aeruginosa were formed by nebulizing a suspension of seaweed sodium-alginate and P. aeruginosa into a calcium solution. The alginate bead method of establishing infection was compared to an agar-bead method and proved to be quantitatively similar after 4 weeks. The ability of the two methods to induce formation of precipitins, IgA and IgG antibodies against P. aeruginosa antigens, including outer membrane proteins, flagella, exoenzymes and alginate, was assessed by crossed immunoelectrophoresis, enzyme-linked immunosorbent assay and immunoblotting. The two methods of inducing infection were comparable and infected rats had significantly higher antibody response than rats inoculated with sterile beads. We suggest that the alginate bead model closely resembles the later stages of CF-lung infection and that it offers the theoretical advantage of using a substance which is chemically similar to the alginate produced in vivo by P. aeruginosa.

Topics: Agar; Alginates; Animals; Antigens, Bacterial; Disease Models, Animal; Female; Microspheres; Pneumonia; Pseudomonas aeruginosa; Pseudomonas Infections; Rats; Rats, Inbred Strains

Isolation, identification and drug sensitivity assay of microorganisms from pathological materials of 177 patients with nonspecific diseases of the lungs, mainly pneumonia, were performed on blood and selective "chocolate" agars by using Baktofok-MK, a new dry nutrient basis developed by the authors. Blood and "chocolate" agars based on the Hottinger's hydrolysate were used as the control media. It was shown that with the quantitative procedure for inoculating the pathological material, the experimental media based on Baktofok-MK were much more sensitive to growth properties that the control media. That made it possible to detect larger numbers of etiologically important microbial species on the blood agar and to isolate clinical strains of Hemophilus spp. from a larger number of specimens on the "chocolate" agar.

Topics: Agar; Anti-Bacterial Agents; Bronchoalveolar Lavage Fluid; Culture Media; Haemophilus Infections; Haemophilus influenzae; Humans; In Vitro Techniques; Pneumonia; Pneumonia, Pneumococcal; Sputum; Streptococcus pneumoniae

Topics: Agar; Animals; Genetic Variation; Humans; Klebsiella; Mice; Pneumonia; Sputum; Virulence

Topics: Agar; Animals; Bacteriolysis; Drug Interactions; Drug Therapy, Combination; Heparin; Immunodiffusion; Injections, Intramuscular; Penicillins; Pneumonia; Rabbits; Streptococcus; Time Factors

Topics: Agar; Animals; Blood; Histological Techniques; Histoplasma; Histoplasmosis; Horse Diseases; Horses; Lung; Male; Pneumonia; Sudan

Topics: Agar; Electrophoresis; Gels; Humans; Methods; Neoplasms; Phospholipids; Pneumonia; Rheumatic Diseases

Topics: Adult; Agar; Amidohydrolases; Bacteria; Cephaloridine; Cephalothin; Culture Media; Humans; Injections, Intramuscular; Injections, Intravenous; Lung Abscess; Male; Methods; Middle Aged; Pneumonia; Staphylococcus

Topics: Adult; Agar; Aged; Anemia; Blood Proteins; Breast Neoplasms; Bronchitis; Colitis; Electrophoresis; Female; Gels; Humans; Infections; Inflammation; Male; Middle Aged; Neoplasms; Orchitis; Pneumonia; Testicular Neoplasms

Topics: Agar; Animals; Anti-Bacterial Agents; Antibiotics, Antitubercular; Chloramphenicol; Drug Resistance, Microbial; Erythrocytes; Erythromycin; Guinea Pigs; Hemolysin Proteins; Horses; Humans; In Vitro Techniques; Mycoplasma; Mycoplasma pneumoniae; Penicillin V; Penicillins; Pneumonia; Pneumonia, Viral; Research; Tetracycline