agar and Periodontal-Pocket

Aggregatibacter actinomycetemcomitans is a pathogen in oral and nonoral infections. Detection and quantification of this pathogen can be performed using selective culture techniques. The aim of this study was to establish the efficacy of two known selective media in their ability to select and support the growth of A. actinomycetemcomitans.. Trypticase soy bacitracin vancomycin (TSBV) medium and brain-heart infusion agar with vancomycin (Dentaid-1), as well as a modified Dentaid-1 medium (in which the brain-heart infusion agar was substituted with brain-heart infusion broth), were compared. Two-hundred and eighteen clinical samples were used to establish the recovery rate, the number of colony-forming units (CFUs) of A. actinomycetemcomitans as well as the total number of CFUs on the three different types of medium. In addition, the numbers of gram-negative aerobic rods and yeasts were determined.. Both types of Dentaid-1 medium showed a higher recovery of A. actinomycetemcomitans compared with TSBV. However, these differences did not reach statistical significance. The total number of CFUs of A. actinomycetemcomitans recovered was significantly higher on Dentaid-1 compared with TSBV (p = 0.029). The mean number of gram-negative aerobic rods recovered was statistically higher on both types of Dentaid-1 medium in comparison with TSBV. Low numbers of yeasts were recovered occasionally on all test plates.. Dentaid-1 is a low-cost effective alternative to TSBV for the isolation and growth of A. actinomycetemcomitans from clinical samples, such as dental plaque, which contain a complex microflora.

Topics: Agar; Aggregatibacter actinomycetemcomitans; Aggressive Periodontitis; Anti-Bacterial Agents; Bacterial Load; Bacteriological Techniques; Caseins; Chronic Periodontitis; Culture Media; Gingival Hemorrhage; Gram-Negative Aerobic Rods and Cocci; Humans; Periodontal Pocket; Protein Hydrolysates; Vancomycin; Yeasts

The aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. From each subject 16-20 samples were taken using paper points. Pooled sample after 60 s. mixing was serially diluted in reduced BHI. For total cell counts and for the isolation of black pigmented anaerobes Brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without Kanamycin-Vancomycin mixture and BM agar plates were used. For isolation of A. actinomycetemcomitans TSBV agar plates were used. Cultures were incubated in anaerobic chamber at 37 degrees C for 7 days and TSBV agar plates in an atmosphere of 95% air-5% CO2 at 37 degrees C for 5 days. Microorganisms were identified by Gram staining, colony morphology, fluorescence in UV-light, haemagglutination of 3% sheep erythrocytes, fermentation of sugars, production of indole, urease (API 20A), specific enzymes (Rapid ID 32A). Twenty seven subjects with clinically recognized periodontitis were examined. Microorganisms important in periodontitis were isolated from periodontal pockets of almost all examined subjects. The number of bacteria obtained from the sample of one patient ranged from 1 x 10(4) CFU/ml to 3,6 x 10(6) CFU/ml. Porphyromonas gingivalis was identified in the samples taken from 17 patients, Prevotella intermedia-19, Actinobacillus actinomycetemcomitans -11, Fusobacterium nucleatum-9, Peptostreptococcus spp.-22.

Topics: Adult; Agar; Bacteria, Aerobic; Bacteria, Anaerobic; Culture Media; Humans; Middle Aged; Periodontal Pocket; Species Specificity

Following long-term periodontal treatment with tetracycline a superinfection with Candida may arise. The reduced environment and the serum transudate of the periodontal pocket may promote such infection. The present in vitro study was performed to ascertain whether yeast-mycelium transformation in a fresh periodontal isolate was promoted under anaerobic conditions and in the presence of serum. C. albicans, isolated from a patient with tetracycline-treated refractory periodontitis, was cultured anaerobically or aerobically on TSBV or Sabouraud's dextrose agar at 29 degrees C or 37 degrees C for 72 h, with the pH of the medium being 5.6 or 7.2. TSBV medium was also tested with its horse serum or yeast extract removed. Mycelial growth was recorded visually and by stereo and scanning electron microscopy. Anaerobic culture at 29 degrees C or 37 degrees C on TSBV provided abundant mycelium at both pHs. After aerobic culture the mycelial phase was less pronounced and more abundant at pH 7.2 than at 5.6. TSBV without serum or yeast extract yielded more mycelium after anaerobic than after aerobic culture, although less than when both components were included. Sabouraud's medium provided sparse mycelium after anaerobic culture irrespective of the pH, and no mycelium after aerobic culture.

Topics: Aerobiosis; Agar; Anaerobiosis; Candida albicans; Colony Count, Microbial; Gingival Crevicular Fluid; Humans; Hydrogen-Ion Concentration; Periodontal Pocket; Spores, Fungal; Superinfection; Temperature; Tetracycline