agar and Peptic-Ulcer

Rapid urease tests (RUTs) are used commonly as a convenient method to detect Helicobacter pylori infection. New rapid tests have been commercially available with promotional literature suggesting enhanced utility. We compared CLOtest to a new reagent strip RUT, PyloriTek.. Gastric antral mucosal biopsy specimens were obtained from 102 patients for comparison between CLOtest and PyloriTek (204 specimens). Biopsy specimens obtained from a nearby area were stained using the Genta stain for determination of H. pylori status. The RUT to be used first was selected randomly.. Sixty-five of the 102 patients had peptic ulcer disease, two had gastric cancer, and 35 had dyspepsia; 61 patients had active H. pylori infection. There were one false-negative and three false-positive CLOtest results, compared with one false-negative and 13 false-positive PyloriTek results (p < 0.02 for incorrect categorization with PyloriTek). Sensitivity and specificity were 98 and 92% compared with 98 and 68% for CLOtest and PyloriTek, respectively. An erroneous categorization of H. pylori status occurred in 3.9% (95% confidence interval [CI]: 1-9.7%) with CLOtest compared with 13.7% (95% CI: 7.7 -22%) with PyloriTek. When the PyloriTek was scored at 1 h (0-1 h) after obtaining the specimen, the accuracy improved; erroneous categorization of H. pylori status occurred in only 2.9% (95% CI: 0.6-8.3%).. Used according to manufacturer instructions, the new reagent strip RUT PyloriTek has too many false-positive results for use in a clinical situation. In contrast, when the test was interpreted within 1 h, accuracy was comparable to that of CLOtest.

Topics: Adult; Agar; Aged; Biopsy; Coloring Agents; Confidence Intervals; Dyspepsia; False Negative Reactions; False Positive Reactions; Female; Gastric Mucosa; Gastroesophageal Reflux; Helicobacter Infections; Helicobacter pylori; Humans; Male; Middle Aged; Peptic Ulcer; Prospective Studies; Pyloric Antrum; Reagent Strips; Sensitivity and Specificity; Stomach Neoplasms; Urease

Ulcer-associated dyspepsia is caused by infection with Helicobacter pylori. H. pylori is linked to a majority of peptic ulcers. Antibiotic treatment does not always inhibit or kill H. pylori with potential for antibiotic resistance. The objective of this study was to determine the potential for using phenolic phytochemical extracts to inhibit H. pylori in a laboratory medium. Our approach involved the development of a specific phenolic profile with optimization of different ratios of extract mixtures from oregano and cranberry. Subsequently, antimicrobial activity and antimicrobial-linked urease inhibition ability were evaluated. The results indicated that the antimicrobial activity was greater in extract mixtures than in individual extracts of each species. The results also indicate that the synergistic contribution of oregano and cranberry phenolics may be more important for inhibition than any species-specific phenolic concentration. Further, based on plate assay, the likely mode of action may be through urease inhibition and disruption of energy production by inhibition of proline dehydrogenase at the plasma membrane.

Topics: Agar; Bacteriological Techniques; Helicobacter Infections; Helicobacter pylori; Humans; Origanum; Peptic Ulcer; Phytotherapy; Plant Extracts; Proline; Urease; Vaccinium macrocarpon

Topics: Agar; Alkaline Phosphatase; Blood Group Antigens; Butyrylcholinesterase; Electrophoresis, Starch Gel; Haptoglobins; Humans; Isoenzymes; Pedigree; Peptic Ulcer

Topics: Agar; Anemia; Blind Loop Syndrome; Body Weight; Diet; Escherichia coli; Feces; Female; Gastrectomy; Gastroenterostomy; Hemoglobins; Humans; Indican; Intestine, Small; Iron; Lipids; Male; Peptic Ulcer; Postoperative Complications; Tryptophan; Vitamin B 12

Topics: Agar; Electrophoresis; Gastric Juice; Gels; Humans; Hydrogen-Ion Concentration; Male; Pepsin A; Peptic Ulcer

Topics: Agar; Electrophoresis; Enzymes; Gastric Juice; Gastric Mucosa; Gastritis; Gels; Humans; Isoenzymes; Pepsin A; Peptic Ulcer; Peptide Hydrolases