agar and Mycobacterium-avium-intracellulare-Infection

agar has been researched along with Mycobacterium-avium-intracellulare-Infection* in 1 studies

Other Studies

1 other study(ies) available for agar and Mycobacterium-avium-intracellulare-Infection

Article	Year
Growth on blood agar discriminates Mycobacterium avium and Mycobacterium intracellulare. Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2003, Volume: 9, Issue:10 Mycobacterium intracellulare and Mycobacterium avium (MAC) require specialized culture and identification procedures. To simplify the diagnosis, we inoculated reference strains, and 85 M. avium and 12 M. intracellulare clinical isolates, on egg-based and sheep blood agar. After 5 days of culture, there were significantly more colonies on sheep blood than on egg-based agar for M. avium (ratio: 250.5 +/- 209) but not for M. intracellulare (ratio: 0.44 +/-0.11). Using a ratio > or = 20, the sensitivity of the identification of an MAC isolate as M. avium was 97.65%, the specificity was 100%, and the positive predictive value was 100%. Differential growth on egg-based and blood agar is an aid to the identification of MAC isolates. Topics: Agar; Colony Count, Microbial; Culture Media; DNA Probes; DNA, Bacterial; Humans; Mycobacterium avium; Mycobacterium avium Complex; Mycobacterium avium-intracellulare Infection; Nucleic Acid Hybridization; RNA, Ribosomal, 16S	2003

Article

Year

Growth on blood agar discriminates Mycobacterium avium and Mycobacterium intracellulare.

Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 2003, Volume: 9, Issue:10

Mycobacterium intracellulare and Mycobacterium avium (MAC) require specialized culture and identification procedures. To simplify the diagnosis, we inoculated reference strains, and 85 M. avium and 12 M. intracellulare clinical isolates, on egg-based and sheep blood agar. After 5 days of culture, there were significantly more colonies on sheep blood than on egg-based agar for M. avium (ratio: 250.5 +/- 209) but not for M. intracellulare (ratio: 0.44 +/-0.11). Using a ratio > or = 20, the sensitivity of the identification of an MAC isolate as M. avium was 97.65%, the specificity was 100%, and the positive predictive value was 100%. Differential growth on egg-based and blood agar is an aid to the identification of MAC isolates.

Topics: Agar; Colony Count, Microbial; Culture Media; DNA Probes; DNA, Bacterial; Humans; Mycobacterium avium; Mycobacterium avium Complex; Mycobacterium avium-intracellulare Infection; Nucleic Acid Hybridization; RNA, Ribosomal, 16S

2003