agar and Multiple-Myeloma

Topics: Acute Disease; Agar; Animals; B-Lymphocytes; Cells, Cultured; Chronic Disease; Colony-Forming Units Assay; Humans; Leukemia, Lymphoid; Methylcellulose; Multiple Myeloma

Topics: Agar; Amino Acid Sequence; Animals; Bence Jones Protein; Chemical Phenomena; Chemistry; Electrophoresis; gamma-Globulins; Humans; Immunoglobulin G; Multiple Myeloma

Topics: Agar; Aged; Alkylating Agents; Anemia, Hemolytic; Blood Coagulation Disorders; Blood Protein Disorders; Blood Viscosity; Chlorambucil; Clinical Trials as Topic; Electrophoresis; Female; gamma-Globulins; Hemolysis; Humans; Immunoelectrophoresis; Immunoglobulin G; Immunoglobulin M; Male; Middle Aged; Multiple Myeloma; Prednisone; Waldenstrom Macroglobulinemia

RWJ-241947 (MCC-555) is a novel peroxisome proliferator-activated receptor-gamma ligand of the thiazolidinedione class that was recently developed as an antidiabetic drug with unique properties. Some thiazolidinediones have anticancer activity against solid and hematological malignancies; the anticancer potency of RWJ-241947 has not been examined. We, therefore, investigated these effects in vitro and in vivo either alone or in combination with other compounds.. Tumor growth was examined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, soft agar colony assay in vitro, and xenografts in nude mice. Its effects on cell cycle, differentiation, and apoptosis were examined.. In vitro studies using various solid and hematological tumor cell lines showed that RWJ-241947 had antiproliferative activity against prostate cancer cells, with the strongest effect against the androgen-independent PC-3 prostate cancer cells. It increased expression of cyclin-dependent kinase inhibitor p21(WAF1), deceased cyclin E, and induced apoptosis in PC-3 cells. It increased E-cadherin and lowered protein expression of prostate-specific antigen without down-regulating the androgen receptor in androgen-dependent LNCaP prostate cancer cells. Reporter gene assays showed that this peroxisome proliferator-activated receptor-gamma ligand inhibited androgen activation of the androgen receptor response elements of the prostate-specific antigen gene. Remarkably, in vivo treatment of male beige/nude/X-linked immunodeficient (BNX) mice with RWJ-241947 profoundly suppressed growth of PC-3 prostate cancer xenografts with prominent apoptosis, as well as fibrosis, including inflammatory and giant cell reaction in the remaining tumor tissue. Notably, the experimented mice had a significantly decreased cholesterol. In addition, we studied the combination of arsenic trioxide (As2O3), which is used in the treatment of multiple myeloma, and RWJ-241947; these two reagents together prominently inhibited proliferation and caused apoptosis of multiple myeloma cells.. RWJ-241947 has surprisingly potent antiproliferative effects against prostate cancer cells in vivo, and it enhances the antitumor activity of As2O3 against myeloma cells. Small, well-defined clinical studies using RWJ-241947 are in order for these cancers.

Topics: Agar; Animals; Antineoplastic Agents; Apoptosis; Arsenic Trioxide; Arsenicals; Blotting, Western; Caspases; CD36 Antigens; Cell Cycle; Cell Differentiation; Cell Division; Cell Line, Tumor; Cell Survival; Genetic Linkage; Humans; Immunologic Deficiency Syndromes; Ligands; Luciferases; Male; Mice; Multiple Myeloma; Oxides; Prostatic Neoplasms; Receptors, Cytoplasmic and Nuclear; Tetrazolium Salts; Thiazoles; Thiazolidinediones; Time Factors; Transcription Factors; Transfection; U937 Cells; X Chromosome

73 bone marrow samples from a total of 50 patients with multiple myeloma (MM) were tested for interferon (IFN) sensitivity in the human tumour colony assay (HTCA). 16 evaluable samples were obtained from untreated patients, 10 from patients during melphalan and prednisone treatment and 6 from patients on IFN treatment or after withdrawal of IFN. The sensitivity to IFN was individually distributed between the bone marrow samples from patients of all 3 groups and a tendency towards IFN resistance could be found in serial assays from patients during MP-treatment and after IFN withdrawal. Stimulation of growth was found in 31% of the cultures, usually at lower doses of IFN (10-100 U/ml) while higher doses (400-4000 U/ml) inhibited growth in 75% of the assays.

Topics: Agar; Bone Marrow Cells; Cells, Cultured; Colony-Forming Units Assay; Culture Media; Humans; Interferon Type I; Multiple Myeloma; Neoplastic Stem Cells

Topics: Agar; Antineoplastic Agents; Cell Survival; Clone Cells; Colony-Forming Units Assay; Culture Techniques; DNA Replication; Drug Evaluation, Preclinical; Humans; Immunoglobulin A; Immunoglobulin G; Interferon Type I; Leukemia, Myeloid, Acute; Multiple Myeloma; Tumor Stem Cell Assay

Clonogenic assays have been widely adopted for the investigation of hematopoietic and human tumor stem cell biology. Inasmuch as specific, whole colonies need to be analyzed morphologically, we used various methods for fixing and embedding individual colonies in situ that allowed macroscopic, light microscopic (LM), immunofluorescence, and transmission electron microscopic (TEM) evaluation of the intact colony. Melanoma colonies stained with Masson's Trichrome, hematoxylin and eosin (H&E), periodic acid-Schiff, Best's carmine, Page-Green method for inclusion bodies, and Snook's reticulum revealed cellular and extracellular components by LM. Ultrastructural studies revealed specific cellular organelles and extracellular components. Immunofluorescence studies demonstrated cell-surface fibronectin, a high molecular weight, adhesive glycoprotein. Myeloma colonies contained a heterogeneous cell population and produced amyloid fibers that were observed by TEM. Fixation and embedding the colonies in agar for TEM has several advantages over centrifugation methods and other conventional techniques for collecting cells in that (a) an entire specific colony can be studied, (b) there is excellent preservation of the cell and its spatial orientation in the colony, and (c) the extracellular matrix (ECM) of the colony is preserved for immunohistochemical analysis.

Topics: Agar; Amyloid; Clone Cells; Extracellular Space; Fibronectins; Fluorescent Antibody Technique; Humans; Melanoma; Methods; Microscopy, Electron; Multiple Myeloma

Topics: Agar; Cellulose; Chromatography, Gel; Cryoglobulins; Electrophoresis; Hot Temperature; Humans; Immunodiffusion; Immunoelectrophoresis; Immunoglobulin A; Immunoglobulin Fragments; Male; Middle Aged; Multiple Myeloma; Oxidation-Reduction; Ultracentrifugation; Viscosity

Topics: Acetates; Adult; Agar; Age Factors; Aged; Alpha-Globulins; Beta-Globulins; Blood Protein Disorders; Blood Protein Electrophoresis; Cellulose; Electrophoresis; Electrophoresis, Paper; gamma-Globulins; Gels; Humans; Middle Aged; Multiple Myeloma; Plasmacytoma; Waldenstrom Macroglobulinemia

The frequency of M-components was studied by agar gel electrophoresis in sera from 807 patients, 467 (57%) females and 340 (43%) males with histologically proven solid malignant neoplasms.M-components were found in the sera of 40 male and 20 female patients. Apart from two known cases of multiple myeloma and one case of Waldenström's macroglobulinaemia, none of the patients were found to be suffering from these diseases. The frequency of M-components increased with age, and this was more evident in males. Twenty-two of 60 patients with M-components did not exhibit abnormalities on immunoelectrophoresis. Of the 35 remaining patients, 27 had an abnormal component of the IgG class, 6 of the IgA and 2 of the IgM class. M-components were found in the sera of patients with a wide variety of neoplasms. There appeared to be no evidence of an increased frequency of M-components in the sera of patients with solid malignant neoplasms compared with normal adult population.

Topics: Adolescent; Adult; Agar; Aged; Child; Electrophoresis; Female; Gastrointestinal Neoplasms; Genital Neoplasms, Female; Humans; Immunoelectrophoresis; Immunoglobulin A; Immunoglobulin G; Immunoglobulin M; Immunoglobulins; Lung Neoplasms; Male; Middle Aged; Multiple Myeloma; Neoplasms; Urinary Bladder Neoplasms; Waldenstrom Macroglobulinemia

Topics: Agar; Animals; Antibodies, Anti-Idiotypic; Antibody Formation; Cell Line; Clone Cells; Culture Media; Electrophoresis, Disc; Fibroblasts; Humans; Immune Sera; Immunodiffusion; Immunoglobulin G; Immunoglobulins; Lens, Crystalline; Lung; Methods; Mice; Multiple Myeloma; Neoplasms, Experimental; Rabbits; Temperature

Topics: Acrylamides; Agar; Animals; Bence Jones Protein; Carbon Isotopes; Chromatography, Gel; Clone Cells; Dinitrophenols; Electrophoresis; Glucosamine; Immunoglobulin A; Immunoglobulins; In Vitro Techniques; Leucine; Mice; Multiple Myeloma; Peptides; Rabbits; Ribosomes; Tritium

Topics: Absorption; Agar; Antigens; Bence Jones Protein; Blood Protein Electrophoresis; Chromatography, Gel; Chromatography, Ion Exchange; gamma-Globulins; Immune Sera; Immunodiffusion; Immunoglobulins; Macroglobulins; Multiple Myeloma; Neoplasm Proteins; Serum Albumin; Waldenstrom Macroglobulinemia

Topics: Agar; Blood Protein Electrophoresis; Diagnosis, Differential; gamma-Globulins; Gels; Humans; Immunoglobulins; Measles; Multiple Myeloma; Subacute Sclerosing Panencephalitis

Topics: Agar; Anemia; Anemia, Hypochromic; Bone Marrow; Bone Marrow Cells; Clone Cells; Culture Media; Culture Techniques; Hodgkin Disease; Humans; Leukemia, Myeloid, Acute; Leukocytes; Multiple Myeloma

Topics: Agar; Chemical Precipitation; Humans; Hydrolysis; Hypergammaglobulinemia; Immunoelectrophoresis; Immunoglobulin G; Lymphoma; Multiple Myeloma; Pectins; Polysaccharides

Topics: Agar; Aspergillosis; Asthma; Celiac Disease; Eczema; Food Hypersensitivity; gamma-Globulins; Hemosiderosis; Humans; Immunodiffusion; Immunoglobulin G; Immunoglobulin M; Larva Migrans, Visceral; Liver Cirrhosis; Lung Diseases; Methods; Multiple Myeloma; Rhinitis, Allergic, Seasonal

Topics: Agar; Bence Jones Protein; Blood Protein Electrophoresis; Chromatography, Gel; gamma-Globulins; Humans; Multiple Myeloma

Topics: Agar; Antigens; Colostrum; Electrophoresis; gamma-Globulins; Humans; Immunochemistry; Immunoelectrophoresis; Molecular Weight; Multiple Myeloma; Saliva; Starch; Ultracentrifugation

Topics: Agar; Animals; Arthritis, Rheumatoid; Beta-Globulins; Complement System Proteins; Hemolysis; Humans; Hydrazines; Immune Sera; Immunoelectrophoresis; Liver Cirrhosis; Multiple Myeloma; Precipitins; Stomach Neoplasms

Topics: Agar; Blood Protein Disorders; Electrophoresis; gamma-Globulins; Gels; Humans; Immunoelectrophoresis; Multiple Myeloma

Topics: Agar; Electrophoresis; Glycoproteins; Humans; Immunoelectrophoresis; In Vitro Techniques; Macroglobulins; Multiple Myeloma; Neoplasm Proteins; Waldenstrom Macroglobulinemia

Topics: Agar; Blood Protein Electrophoresis; Electrophoresis, Agar Gel; Humans; L-Lactate Dehydrogenase; Liver Diseases; Multiple Myeloma; Shock

Topics: Agar; Animals; Blood; Immunoelectrophoresis; Multiple Myeloma; Polysaccharides; Precipitin Tests; Rabbits; Sepharose; Waldenstrom Macroglobulinemia

Topics: Agar; Blood Proteins; Cryoglobulinemia; Multiple Myeloma; Plasma Cells; Serum Globulins; Waldenstrom Macroglobulinemia